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. 1982 Jul;43(1):325–327. doi: 10.1128/jvi.43.1.325-327.1982

Differential effect of nucleoside analog triphosphates on ribonucleotide reductases from uninfected and herpes simplex virus-infected HeLa cells.

K Nakayama, J L Ruth, Y C Cheng
PMCID: PMC256123  PMID: 6287006

Abstract

Effects of the triphosphates of eight pyrimidine nucleoside analogs (5-substituted, 2'-fluoroara-, and acyclonucleosides) and acycloguanosine were examined on the ribonucleotide reductases prepared from uninfected and herpes simplex virus (types 1 and 2)-infected HeLa cells. Of the analogs tested, E-5-propenyl- and E-5-(2-bromovinyl)-dUTP were more potent inhibitors than dTTP of the enzymes from virus-infected cells, whereas only the former compound showed this effect on the uninfected HeLa enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caradonna S. J., Cheng Y. C. Induction of uracil-DNA glycosylase and dUTP nucleotidohydrolase activity in herpes simplex virus-infected human cells. J Biol Chem. 1981 Oct 10;256(19):9834–9837. [PubMed] [Google Scholar]
  2. Chang C. H., Cheng Y. C. Demonstration of two components and association of adenosine diphosphate-cytidine diphosphate reductase from cultured human lymphoblast cells (Molt-4F). Cancer Res. 1979 Feb;39(2 Pt 1):436–442. [PubMed] [Google Scholar]
  3. Chang C. H., Cheng Y. C. Effects of nucleoside triphosphates on human ribonucleotide reductase from Molt-4F cells. Cancer Res. 1979 Dec;39(12):5087–5092. [PubMed] [Google Scholar]
  4. Cheng Y. C., Grill S., Ruth J., Bergstrom D. E. Anti-herpes simplex virus and anti-human cell growth activity of E-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy. Antimicrob Agents Chemother. 1980 Dec;18(6):957–961. doi: 10.1128/aac.18.6.957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Clercq E., Descamps J., De Somer P., Barr P. J., Jones A. S., Walker R. T. (E)-5-(2-Bromovinyl)-2'-deoxyuridine: a potent and selective anti-herpes agent. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2947–2951. doi: 10.1073/pnas.76.6.2947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elford H. L., Freese M., Passamani E., Morris H. P. Ribonucleotide reductase and cell proliferation. I. Variations of ribonucleotide reductase activity with tumor growth rate in a series of rat hepatomas. J Biol Chem. 1970 Oct 25;245(20):5228–5233. [PubMed] [Google Scholar]
  7. Elion G. B., Furman P. A., Fyfe J. A., de Miranda P., Beauchamp L., Schaeffer H. J. Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5716–5720. doi: 10.1073/pnas.74.12.5716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huszar D., Bacchetti S. Partial purification and characterization of the ribonucleotide reductase induced by herpes simplex virus infection of mammalian cells. J Virol. 1981 Feb;37(2):580–588. doi: 10.1128/jvi.37.2.580-588.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Langelier Y., Déchamps M., Buttin G. Aanlysis of dCMP deaminase and CDP reductase levels in hamster cells infected by herpes simplex virus. J Virol. 1978 Jun;26(3):547–553. doi: 10.1128/jvi.26.3.547-553.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ponce de Leon M., Eisenberg R. J., Cohen G. H. Ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected KB cells: properties of the partially purified enzymes. J Gen Virol. 1977 Jul;36(1):163–173. doi: 10.1099/0022-1317-36-1-163. [DOI] [PubMed] [Google Scholar]
  11. Ruth J. L., Cheng Y. C. Nucleoside analogues with clinical potential in antivirus chemotherapy. The effect of several thymidine and 2'-deoxycytidine analogue 5'-triphosphates on purified human (alpha, beta) and herpes simplex virus (types 1, 2) DNA polymerases. Mol Pharmacol. 1981 Sep;20(2):415–422. [PubMed] [Google Scholar]
  12. Thelander L., Reichard P. Reduction of ribonucleotides. Annu Rev Biochem. 1979;48:133–158. doi: 10.1146/annurev.bi.48.070179.001025. [DOI] [PubMed] [Google Scholar]
  13. Watanabe K. A., Reichman U., Hirota K., Lopez C., Fox J. J. Nucleosides. 110. Synthesis and antiherpes virus activity of some 2'-fluoro-2'-deoxyarabinofuranosylpyrimidine nucleosides. J Med Chem. 1979 Jan;22(1):21–24. doi: 10.1021/jm00187a005. [DOI] [PubMed] [Google Scholar]

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