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. 1982 Jul;43(1):365–367. doi: 10.1128/jvi.43.1.365-367.1982

In vitro packaging of bacteriophage T7 DNA requires ATP.

W E Masker
PMCID: PMC256132  PMID: 7109030

Abstract

Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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