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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: Biochemistry. 2007 May 26;46(24):7146–7152. doi: 10.1021/bi700548a

Figure 3.

Figure 3

Separation of CcO subcomplexes by HiTrapQ FPLC anion-exchange chromatography. The left panel depicts the elution of dimeric CcO before (1) and after (2) pressurization at 3 kbar for 2 h. The middle panel depicts the elution of monomeric CcO before (1) and after (2) pressurization at 3 kbar for 2 h. The right panel depicts the elution of monomeric CcO as a function of time at 3 kbar pressure: (1) 0, (2) 20, (3) 30, and (4) 120 min. Elution and gradient conditions are identical to those previously described (18). Nearly identical elution profiles were obtained each time pressure-treated CcO was analyzed (experiment repeated more than a dozen times).