Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1982 Sep;43(3):1138–1142. doi: 10.1128/jvi.43.3.1138-1142.1982

DNA packaging in vitro by an isolated bacteriophage T7 procapsid.

W E Masker, P Serwer
PMCID: PMC256228  PMID: 7143564

Abstract

The results of previous in vivo studies indicate that a DNA-free procapsid (capsid I) packages bacteriophage T7 DNA during infection of Escherichia coli. It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts deficient in capsid proteins.

Full text

PDF
1138

Images in this article

1140Image
on p.1140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Earnshaw W. C., Casjens S. R. DNA packaging by the double-stranded DNA bacteriophages. Cell. 1980 Sep;21(2):319–331. doi: 10.1016/0092-8674(80)90468-7. [DOI] [PubMed] [Google Scholar]
  2. Kerr C., Sadowski P. D. Packaging and maturation of DNA of bacteriophage T7 in vitro. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3545–3549. doi: 10.1073/pnas.71.9.3545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kuemmerle N. B., Masker W. E. In vitro packaging of UV radiation-damaged DNA from bacteriophage T7. J Virol. 1977 Sep;23(3):509–516. doi: 10.1128/jvi.23.3.509-516.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kuhn A. H., Moncany M. L., Kellenberger E., Hausmann R. Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. I. Arrest at the level of DNA packaging. J Virol. 1982 Feb;41(2):657–673. doi: 10.1128/jvi.41.2.657-673.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Masker W. E., Kuemmerle N. B., Allison D. P. In vitro packaging of bacteriophate T7 DNA synthesized in vitro. J Virol. 1978 Jul;27(1):149–163. doi: 10.1128/jvi.27.1.149-163.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Poteete A. R., Jarvik V., Botstein D. Encapsulation of phage P22 DNA in vitro. Virology. 1979 Jun;95(2):550–564. doi: 10.1016/0042-6822(79)90508-7. [DOI] [PubMed] [Google Scholar]
  7. Richardson C. C., Romano L. J., Kolodner R., LeClerc J. E., Tamanoi F., Engler M. J., Dean F. B., Richardson D. S. Replication of bacteriophage T7 DNA by purified proteins. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):427–440. doi: 10.1101/sqb.1979.043.01.049. [DOI] [PubMed] [Google Scholar]
  8. Roeder G. S., Sadowski P. D. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage. Virology. 1977 Jan;76(1):263–285. doi: 10.1016/0042-6822(77)90302-6. [DOI] [PubMed] [Google Scholar]
  9. Roeder G. S., Sadowski P. D. Pathways of recombination of bacteriophage T7 DNA in vitro. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1023–1032. doi: 10.1101/sqb.1979.043.01.111. [DOI] [PubMed] [Google Scholar]
  10. Serwer P. A metrizamide-impermeable capsid in the DNA packaging pathway of bacteriophage T7. J Mol Biol. 1980 Mar 25;138(1):65–91. doi: 10.1016/s0022-2836(80)80005-2. [DOI] [PubMed] [Google Scholar]
  11. Serwer P. Fibrous projections from the core of a bacteriophage T7 procapsid. J Supramol Struct. 1979;11(3):321–326. doi: 10.1002/jss.400110307. [DOI] [PubMed] [Google Scholar]
  12. Serwer P. Internal proteins of bacteriophage T7. J Mol Biol. 1976 Nov 5;107(3):271–291. doi: 10.1016/s0022-2836(76)80005-8. [DOI] [PubMed] [Google Scholar]
  13. Serwer P., Pichler M. E. Electrophoresis of bacteriophage T7 and T7 capsids in agarose gels. J Virol. 1978 Dec;28(3):917–928. doi: 10.1128/jvi.28.3.917-928.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Serwer P., Watson R. H. Capsid-DNA complexes in the DNA packaging pathway of bacteriophage T7: characterization of the capsids bound to monomeric and concatemeric DNA. Virology. 1981 Jan 15;108(1):164–176. doi: 10.1016/0042-6822(81)90536-5. [DOI] [PubMed] [Google Scholar]
  15. Serwer P., Watson R. H. Detection of viral capsid-DNA complexes. Prog Clin Biol Res. 1981;64:231–238. [PubMed] [Google Scholar]
  16. Serwer P., Watson R. H. Electrophoresis in density gradients of metrizamide. Anal Biochem. 1981 Jul 1;114(2):342–348. doi: 10.1016/0003-2697(81)90491-7. [DOI] [PubMed] [Google Scholar]
  17. Serwer P., Watson R. H., Hayes S. J. Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid. J Virol. 1982 May;42(2):583–594. doi: 10.1128/jvi.42.2.583-594.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  19. Syvanen M., Yin J. Studies of DNA packaging into the heads of bacteriophage lambda. J Mol Biol. 1978 Dec 15;126(3):333–346. doi: 10.1016/0022-2836(78)90044-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES