Abstract
The protease encoded by the large (B) RNA segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (M) RNA segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (CPsMV, strains Dg and Ark), and from the nepovirus tomato black ring virus. Like M RNA from cowpea mosaic virus, the M RNAs from squash mosaic virus, red clover mottle virus, CPsMV-Dg, and CPsMV-Ark were all translated into two large polypeptides with apparent molecular weights which were different for each virus and even for the two CPsMV strains. Neither the in vitro products from squash mosaic virus, red clover mottle virus, and CPsMV M RNAs nor the in vitro product from tomato black ring virus RNA-2 were processed by the cowpea mosaic virus-encoded protease, indicating that the activity of this enzyme is highly specific.
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- Daubert S. D., Bruening G., Najarian R. C. Protein bound to the genome RNAs of cowpea mosaic virus. Eur J Biochem. 1978 Dec 1;92(1):45–51. doi: 10.1111/j.1432-1033.1978.tb12721.x. [DOI] [PubMed] [Google Scholar]
- Franssen H., Goldbach R., Broekhuijsen M., Moerman M., van Kammen A. Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells. J Virol. 1982 Jan;41(1):8–17. doi: 10.1128/jvi.41.1.8-17.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldbach R. W., Schilthuis J. G., Rezelman G. Comparison of in vivo and in vitro translation of cowpea mosaic virus RNAs. Biochem Biophys Res Commun. 1981 Mar 16;99(1):89–94. doi: 10.1016/0006-291x(81)91716-2. [DOI] [PubMed] [Google Scholar]
- Goldbach R., Rezelman G., Zabel P., van Kammen A. Expression of the Bottom-Component RNA of Cowpea Mosaic Virus: Evidence that the 60-Kilodalton VPg Precursor Is Cleaved into Single VPg and a 58-Kilodalton Polypeptide. J Virol. 1982 May;42(2):630–635. doi: 10.1128/jvi.42.2.630-635.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaspars E. M. Plant viruses with a multipartite genome. Adv Virus Res. 1974;19:37–149. doi: 10.1016/s0065-3527(08)60659-4. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
- Pelham H. R. Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology. 1979 Jul 30;96(2):463–477. doi: 10.1016/0042-6822(79)90104-1. [DOI] [PubMed] [Google Scholar]
- Reijnders L., Aalbers A. M., van Kammen A., Thuring R. W. Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions. Virology. 1974 Aug;60(2):515–521. doi: 10.1016/0042-6822(74)90345-6. [DOI] [PubMed] [Google Scholar]
- Rezelman G., Goldbach R., Van Kammen A. Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. J Virol. 1980 Nov;36(2):366–373. doi: 10.1128/jvi.36.2.366-373.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zabel P., Moerman M., van Straaten F., Goldbach R., van Kammen A. Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide. J Virol. 1982 Mar;41(3):1083–1088. doi: 10.1128/jvi.41.3.1083-1088.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]