Table 1.
Molecular weight, lysine (K) and phenylalanine (F) content, transition temperature (Tt) and fusion ΔTt parameters for purified ELPs and Trx-ELPs at 25 μM in PBS (N/A*: not available)
| Purified ELPs |
Purified Trx-ELPs |
fusion ΔTt parameter | |||||
|---|---|---|---|---|---|---|---|
| ELPs | # pentapeptides | #K or F residues | Observed MW (kDa) | Tt (°C) | Observed MW (kDa) | Tt (°C) | |
| [KV7F] | 9 | 1 | N/A* | N/A* | 18.5 | >90.0 | N/A* |
| 18 | 2 | 8.4 | >90.0 | 22.2 | 73.1 | >-16.9 | |
| 36 | 4 | 15.8 | 48.0 | 29.7 | 42.1 | -5.9 | |
| 72 | 8 | 31.0 | 35.7 | 44.9 | 34.0 | -1.7 | |
| 144 | 16 | 61.1 | 26.1 | 74.9 | 28.4 | 2.3 | |
| [KV2F] | 8 | 2 | N/A* | N/A* | 18.1 | >90.0 | N/A* |
| 16 | 4 | 7.7 | >90.0 | 21.5 | >90.0 | N/A* | |
| 32 | 8 | 14.5 | >90.0 | 28.4 | 67.4 | >-22.6 | |
| 64 | 16 | 28.4 | 60.7 | 42.1 | 43.1 | -17.6 | |
| 128 | 32 | 55.7 | 42.4 | 69.6 | 37.3 | -5.1 | |