Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1982 Oct;44(1):263–268. doi: 10.1128/jvi.44.1.263-268.1982

Two colinear and spliced viral transcripts are present in non-virus-producing benign and malignant neoplasms induced by the shope (rabbit) papilloma virus.

M Nasseri, F O Wettstein, J G Stevens
PMCID: PMC256261  PMID: 6292489

Abstract

The nature of Shope virus-specific RNA was investigated in non-virus-producing Shope (rabbit) papilloma virus-induced benign and malignant domestic rabbit tumors and in a cell line derived from the VX-7 transplantable carcinoma. RNA transfer (Northern) blot analysis of polyadenylated RNA isolated from whole cell extracts of all three sources was resolved into two major bands of 1.3 and 2.0 kilobases. Two additional minor bands of 3.5 and 4.8 kilobases could be seen in some analyses. RNA of the VX-7 cell line was further separated into nuclear, cytoplasmic, and polysomal fractions. The cytoplasmic fraction only contained the 1.3- and 2.0-kilobase species, and virus-specific RNA was found to be associated with polysomes. The two major transcripts present in VX-7 cells were mapped by hybridization of RNA transfer blots with subgenomic probes, and the results indicated that both transcripts are spliced and are most likely colinear. Our results are consistent with the suggestion that the two viral transcripts are necessary for induction and maintenance of the neoplasms.

Full text

PDF
263

Images in this article

265Image
on p.265
265Image
on p.265
266Image
on p.266

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bantle J. A., Maxwell I. H., Hahn W. E. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976 May 7;72:413–427. doi: 10.1016/0003-2697(76)90549-2. [DOI] [PubMed] [Google Scholar]
  2. Berger S. L., Birkenmeier C. S. Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry. 1979 Nov 13;18(23):5143–5149. doi: 10.1021/bi00590a018. [DOI] [PubMed] [Google Scholar]
  3. Berk A. J., Lee F., Harrison T., Williams J., Sharp P. A. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell. 1979 Aug;17(4):935–944. doi: 10.1016/0092-8674(79)90333-7. [DOI] [PubMed] [Google Scholar]
  4. Breitburd F., Favre M., Zoorob R., Fortin D., Orth G. Detection and characterization of viral genomes and search for tumoral antigens in two hamster cell lines derived from tumors induced by bovine papillomavirus type 1. Int J Cancer. 1981 May 15;27(5):693–702. doi: 10.1002/ijc.2910270517. [DOI] [PubMed] [Google Scholar]
  5. Freese U. K., Schulte P., Pfister H. Papilloma virus-induced tumors contain a virus-specific transcript. Virology. 1982 Feb;117(1):257–261. doi: 10.1016/0042-6822(82)90525-6. [DOI] [PubMed] [Google Scholar]
  6. Hulse J. L., Wettstein F. O. Two separable cytoplasmic pools of native ribosomal subunits in chick embryo tissue culture cells. Biochim Biophys Acta. 1972 May 10;269(2):265–275. doi: 10.1016/0005-2787(72)90436-4. [DOI] [PubMed] [Google Scholar]
  7. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McVay P., Fretz M., Wettstein F., Stevens J., Ito Y. Integrated shope virus DNA is present and transcribed in the transplantable rabbit tumour Vx-7. J Gen Virol. 1982 Jun;60(Pt 2):271–278. doi: 10.1099/0022-1317-60-2-271. [DOI] [PubMed] [Google Scholar]
  9. Moar M. H., Campo M. S., Laird H. M., Jarrett W. F. Unintegrated viral DNA sequences in a hamster tumor induced by bovine papilloma virus. J Virol. 1981 Sep;39(3):945–949. doi: 10.1128/jvi.39.3.945-949.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moar M. H., Campo M. S., Laird H., Jarrett W. F. Persistence of non-integrated viral DNA in bovine cells transformed in vitro by bovine papillomavirus type 2. Nature. 1981 Oct 29;293(5835):749–751. doi: 10.1038/293749a0. [DOI] [PubMed] [Google Scholar]
  11. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  12. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  13. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Stevens J. G., Wettstein F. O. Multiple copies of Shope virus DNA are present in cells of benign and malignant non-virus-producing neoplasms. J Virol. 1979 Jun;30(3):891–898. doi: 10.1128/jvi.30.3.891-898.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wettstein F. O., Stevens J. G. Transcription of the viral genome in papillomas and carcinomas induced by the Shope virus. Virology. 1981 Mar;109(2):448–451. doi: 10.1016/0042-6822(81)90517-1. [DOI] [PubMed] [Google Scholar]
  19. Wettstein F. O., Stevens J. G. Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some. Proc Natl Acad Sci U S A. 1982 Feb;79(3):790–794. doi: 10.1073/pnas.79.3.790. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES