Abstract
A stable hybridoma cell line, IIG8-203-2, that secretes a monoclonal antibody of the immunoglobulin subclass M was obtained by fusion of mouse myeloma cells with spleen cells of mice that had been immunized with the viral polypeptide VP2 of simian virus 40. The monoclonal antibody recognizes viral polypeptides that migrate with VP2 polypeptides in a sodium dodecyl sulfate-polyacrylamide gel. It also recognizes two intracellular polypeptides (29,000 and 37,000 daltons) in a detergent-insoluble fraction extracted 30 h after virus infection of TC7 cells.
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- Anderson C. W., Gesteland R. F. Pattern of protein synthesis in monkey cells infected by simian virus 40. J Virol. 1972 May;9(5):758–765. doi: 10.1128/jvi.9.5.758-765.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cervera M., Dreyfuss G., Penman S. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells. Cell. 1981 Jan;23(1):113–120. doi: 10.1016/0092-8674(81)90276-2. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Estes M. K., Huang E. S., Pagano J. S. Structural polypeptides of simian virus 40. J Virol. 1971 May;7(5):635–641. doi: 10.1128/jvi.7.5.635-641.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fey G., Hirt B. Fingerprints of polyoma virus proteins and mouse histones. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):235–241. doi: 10.1101/sqb.1974.039.01.030. [DOI] [PubMed] [Google Scholar]
- Fischer H., Sauer G. Identification of virus-induced proteins in cells productively infected with simian virus 40. J Virol. 1972 Jan;9(1):1–9. doi: 10.1128/jvi.9.1.1-9.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasamatsu H., Flory P. J., Jr Synthesis of the SV40 viral polypeptide Vp1 during infection. Virology. 1978 May 15;86(2):344–353. doi: 10.1016/0042-6822(78)90075-2. [DOI] [PubMed] [Google Scholar]
- Kasamatsu H., Nehorayan A. Intracellular localization of viral polypeptides during simian virus 40 infection. J Virol. 1979 Nov;32(2):648–660. doi: 10.1128/jvi.32.2.648-660.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasamatsu H., Nehorayan A. Vp1 affects intracellular localization of Vp3 polypeptide during simian virus 40 infection. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2808–2812. doi: 10.1073/pnas.76.6.2808. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasamatsu H., Shyamala M., Lin W. Host antigens in the centriolar region are induced in SV40-infected TC7 cells: SV40 small-T-function requirement. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):243–252. doi: 10.1101/sqb.1980.044.01.028. [DOI] [PubMed] [Google Scholar]
- Kiehn E. D. Protein metabolism in SV40-infected cells. Virology. 1973 Nov;56(1):313–333. doi: 10.1016/0042-6822(73)90309-7. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Lake R. S., Barban S., Salzman N. P. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40. Biochem Biophys Res Commun. 1973 Sep 18;54(2):640–647. doi: 10.1016/0006-291x(73)91471-x. [DOI] [PubMed] [Google Scholar]
- Ozer H. L. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reddy V. B., Thimmappaya B., Dhar R., Subramanian K. N., Zain B. S., Pan J., Ghosh P. K., Celma M. L., Weissman S. M. The genome of simian virus 40. Science. 1978 May 5;200(4341):494–502. doi: 10.1126/science.205947. [DOI] [PubMed] [Google Scholar]
- Rozenblatt S., Mulligan R. C., Gorecki M., Roberts B. E., Rich A. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2747–2751. doi: 10.1073/pnas.73.8.2747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walter G., Roblin R., Dulbecco R. Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972 Apr;69(4):921–924. doi: 10.1073/pnas.69.4.921. [DOI] [PMC free article] [PubMed] [Google Scholar]




