Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1982 Dec;44(3):794–803. doi: 10.1128/jvi.44.3.794-803.1982

Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins.

D F Stern, B M Sefton
PMCID: PMC256336  PMID: 6294329

Abstract

We examined the synthesis of viral structural proteins in cultured cells infected with the avian coronavirus infectious bronchitis virus. Tryptic peptide mapping was used to determine the structural relationships of the intracellular proteins to the virion polypeptides. Pulse-chase experiments were performed to identify precursors to the virus-specific proteins. We found that the nucleocapsid protein, P51, and the small viral membrane proteins GP31, GP28, and P23 do not undergo post-translational proteolytic processing. In contrast, GP90 and GP84, the two large virion membrane proteins, were found to be produced by cleavage of a single precursor, GP155. This demonstrated that at least one coronavirus mRNA specifies two virion proteins.

Full text

PDF
794

Images in this article

796Image
on p.796
797Image
on p.797
798Image
on p.798
799Image
on p.799
800Image
on p.800
801Image
on p.801

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beemon K., Hunter T. Characterization of Rous sarcoma virus src gene products synthesized in vitro. J Virol. 1978 Nov;28(2):551–566. doi: 10.1128/jvi.28.2.551-566.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Bonner W. M., Stedman J. D. Efficient fluorography of 3H and 14C on thin layers. Anal Biochem. 1978 Aug 15;89(1):247–256. doi: 10.1016/0003-2697(78)90747-9. [DOI] [PubMed] [Google Scholar]
  4. Cavanagh D. Structural polypeptides of coronavirus IBV. J Gen Virol. 1981 Mar;53(Pt 1):93–103. doi: 10.1099/0022-1317-53-1-93. [DOI] [PubMed] [Google Scholar]
  5. Cheley S., Anderson R. Cellular synthesis and modification of murine hepatitis virus polypeptides. J Gen Virol. 1981 Jun;54(Pt 2):301–311. doi: 10.1099/0022-1317-54-2-301. [DOI] [PubMed] [Google Scholar]
  6. DAVID-FERREIRA J. F., MANAKER R. A. AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS. J Cell Biol. 1965 Jan;24:57–78. doi: 10.1083/jcb.24.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ducatelle R., Coussement W., Pensaert M. B., Debouck P., Hoorens J. In vivo morphogenesis of a new porcine enteric coronavirus, CV 777. Arch Virol. 1981;68(1):35–44. doi: 10.1007/BF01315165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duda E., Schlesinger M. J. Alterations in Sindbis viral enbelope proteins by treating BHK cells with glucosamine. J Virol. 1975 Feb;15(2):416–419. doi: 10.1128/jvi.15.2.416-419.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holmes K. V., Doller E. W., Sturman L. S. Tunicamycin resistant glycosylation of coronavirus glycoprotein: demonstration of a novel type of viral glycoprotein. Virology. 1981 Dec;115(2):334–344. doi: 10.1016/0042-6822(81)90115-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lomniczi B. Biological properties of avian coronavirus RNA. J Gen Virol. 1977 Sep;36(3):531–533. doi: 10.1099/0022-1317-36-3-531. [DOI] [PubMed] [Google Scholar]
  11. Lomniczi B., Kennedy I. Genome of infectious bronchitis virus. J Virol. 1977 Oct;24(1):99–107. doi: 10.1128/jvi.24.1.99-107.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lomniczi B., Morser J. Polypeptides of infectious bronchitis virus. I. Polypeptides of the virion. J Gen Virol. 1981 Jul;55(Pt 1):155–164. doi: 10.1099/0022-1317-55-1-155. [DOI] [PubMed] [Google Scholar]
  13. Macnaughton M. R., Madge M. H. The characterisation of the virion RNA of avian infectious bronchitis virus. FEBS Lett. 1977 May 15;77(2):311–313. doi: 10.1016/0014-5793(77)80258-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Massalski A., Coulter-Mackie M., Dales S. Assembly of mouse hepatitis virus strain JHM. Adv Exp Med Biol. 1981;142:111–118. doi: 10.1007/978-1-4757-0456-3_9. [DOI] [PubMed] [Google Scholar]
  15. Rothman J. E., Lodish H. F. Synchronised transmembrane insertion and glycosylation of a nascent membrane protein. Nature. 1977 Oct 27;269(5631):775–780. doi: 10.1038/269775a0. [DOI] [PubMed] [Google Scholar]
  16. Rottier P. J., Horzinek M. C., van der Zeijst B. A. Viral protein synthesis in mouse hepatitis virus strain A59-infected cells: effect of tunicamycin. J Virol. 1981 Nov;40(2):350–357. doi: 10.1128/jvi.40.2.350-357.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rottier P. J., Spaan W. J., Horzinek M. C., van der Zeijst B. A. Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes. J Virol. 1981 Apr;38(1):20–26. doi: 10.1128/jvi.38.1.20-26.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schochetman G., Stevens R. H., Simpson R. W. Presence of infectious polyadenylated RNA in coronavirus avian bronchitis virus. Virology. 1977 Apr;77(2):772–782. doi: 10.1016/0042-6822(77)90498-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sefton B. M., Beemon K., Hunter T. Comparison of the expression of the src gene of Rous sarcoma virus in vitro and in vivo. J Virol. 1978 Dec;28(3):957–971. doi: 10.1128/jvi.28.3.957-971.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sefton B. M. Immediate glycosylation of Sindbis virus membrane proteins. Cell. 1977 Apr;10(4):659–668. doi: 10.1016/0092-8674(77)90099-x. [DOI] [PubMed] [Google Scholar]
  21. Siddell S., Wege H., Barthel A., ter Meulen V. Coronavirus JHM: intracellular protein synthesis. J Gen Virol. 1981 Mar;53(Pt 1):145–155. doi: 10.1099/0022-1317-53-1-145. [DOI] [PubMed] [Google Scholar]
  22. Stern D. F., Burgess L., Sefton B. M. Structural analysis of virion proteins of the avian coronavirus infectious bronchitis virus. J Virol. 1982 Apr;42(1):208–219. doi: 10.1128/jvi.42.1.208-219.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stern D. F., Kennedy S. I. Coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA. J Virol. 1980 Jun;34(3):665–674. doi: 10.1128/jvi.34.3.665-674.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stern D. F., Kennedy S. I. Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. J Virol. 1980 Nov;36(2):440–449. doi: 10.1128/jvi.36.2.440-449.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stern D. F., Sefton B. M. Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. J Virol. 1982 Dec;44(3):804–812. doi: 10.1128/jvi.44.3.804-812.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sturman L. S., Holmes K. V., Behnke J. Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J Virol. 1980 Jan;33(1):449–462. doi: 10.1128/jvi.33.1.449-462.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sturman L. S., Holmes K. V. Characterization of coronavirus II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology. 1977 Apr;77(2):650–660. doi: 10.1016/0042-6822(77)90489-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sturman L. S. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology. 1977 Apr;77(2):637–649. doi: 10.1016/0042-6822(77)90488-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wadey C. N., Westaway E. G. Structural proteins and glycoproteins of infectious bronchitis virus particles labeled during growth in chick embryo cells. Intervirology. 1981;15(1):19–27. doi: 10.1159/000149210. [DOI] [PubMed] [Google Scholar]
  30. Watkins H., Reeve P., Alexander D. J. The ribonucleic acid of infectious bronchitis virus. Arch Virol. 1975;47(3):279–286. doi: 10.1007/BF01317815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Welch W. J., Sefton B. M., Esch F. S. Amino-terminal sequence analysis of alphavirus polypeptides. J Virol. 1981 Jun;38(3):968–972. doi: 10.1128/jvi.38.3.968-972.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wirth D. F., Katz F., Small B., Lodish H. F. How a single Sindbis virus mRNA directs the synthesis of one soluble protein and two integral membrane glycoproteins. Cell. 1977 Feb;10(2):253–263. doi: 10.1016/0092-8674(77)90219-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES