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. 1982 Dec;44(3):893–899. doi: 10.1128/jvi.44.3.893-899.1982

Identification and characterization of a DNase induced by Epstein-Barr virus.

R S Tan, A K Datta, Y C Cheng
PMCID: PMC256348  PMID: 6294337

Abstract

The diterpene ester promoter of mouse skin tumors, 12-O-tetradecanoyl-phorbol-13-acetate, induced a DNase activity in the Epstein-Barr virus-producer cell line P3HR-1. The elution patterns of the enzyme from DEAE-cellulose, phosphocellulose, and DNA-cellulose columns were different from virus-associated DNA polymerase activity. The partially purified activity could be neutralized to the extent of 90% by sera of patients with nasopharyngeal carcinoma. Purified immunoglobulin G from sera of nasopharyngeal carcinoma patients inhibited this enzyme and that obtained from superinfected Raji cells to the same extent. The partially purified enzyme preferred native DNA as a substrate over denatured DNA and 3'-terminally labeled activated calf thymus DNA. The activity was inhibited by high ionic strength. Phosphonoformic acid did not have any effect on this enzyme activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheng Y. C., Chen J. Y., Glaser R., Henle W. Frequency and levels of antibodies to Epstein-Barr virus-specific DNase are elevated in patients with nasopharyngeal carcinoma. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6162–6165. doi: 10.1073/pnas.77.10.6162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheng Y. C., Chen J. Y., Hoffmann P. J., Glaser R. Studies on the activity of DNase associated with the replication of the Epstein-Barr virus. Virology. 1980 Jan 30;100(2):334–338. doi: 10.1016/0042-6822(80)90524-3. [DOI] [PubMed] [Google Scholar]
  3. Clough W. An endonuclease isolated from Epstein-Barr virus-producing human lymphoblastoid cells. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6194–6198. doi: 10.1073/pnas.77.10.6194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clough W. Deoxyribonuclease activity found in Epstein--Barr virus producing lymphoblastoid cells. Biochemistry. 1979 Oct 16;18(21):4517–4521. doi: 10.1021/bi00588a009. [DOI] [PubMed] [Google Scholar]
  5. Datta A. K., Feighny R. J., Pagano J. S. Induction of Epstein-Barr virus-associated DNA polymerase by 12-O-tetradecanoylphorbol-13-acetate. Purification and characterization. J Biol Chem. 1980 Jun 10;255(11):5120–5125. [PubMed] [Google Scholar]
  6. Datta A. K., Hood R. E. Mechanism of inhibition of Epstein-Barr virus replication by phosphonoformic acid. Virology. 1981 Oct 15;114(1):52–59. doi: 10.1016/0042-6822(81)90251-8. [DOI] [PubMed] [Google Scholar]
  7. Derse D., Cheng Y. C. Herpes simplex virus type I DNA polymerase. Kinetic properties of the associated 3'-5' exonuclease activity and its role in araAMP incorporation. J Biol Chem. 1981 Aug 25;256(16):8525–8530. [PubMed] [Google Scholar]
  8. Feighny R. J., Henry B. E., 2nd, Pagano J. S. Epstein-Barr virus-induced deoxynuclease and the reutilization of host-cell DNA degradation products in viral DNA replication. Virology. 1981 Dec;115(2):395–400. doi: 10.1016/0042-6822(81)90121-5. [DOI] [PubMed] [Google Scholar]
  9. Francke B., Moss H., Timbury M. C., Hay J. Alkaline DNase activity in cells infected with a temperature-sensitive mutant of herpes simplex virus type 2. J Virol. 1978 May;26(2):209–213. doi: 10.1128/jvi.26.2.209-213.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodman S. R., Prezyna C., Benz W. C. Two Epstein-Barr virus-associated DNA polymerase activities. J Biol Chem. 1978 Dec 10;253(23):8617–8628. [PubMed] [Google Scholar]
  11. Graw J., Schlaeger E. J., Knippers R. A lymphocyte ATP-dependent deoxyribonuclease. Isolation and properties. J Biol Chem. 1981 Dec 25;256(24):13207–13212. [PubMed] [Google Scholar]
  12. Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoffmann P. J., Cheng Y. C. DNase induced after infection of KB cells by herpes simplex virus type 1 or type 2. II. Characterization of an associated endonuclease activity. J Virol. 1979 Nov;32(2):449–457. doi: 10.1128/jvi.32.2.449-457.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hoffmann P. J., Cheng Y. C. The deoxyribonuclease induced after infection of KB cells by herpes simplex virus type 1 or type 2. I. Purification and characterization of the enzyme. J Biol Chem. 1978 May 25;253(10):3557–3562. [PubMed] [Google Scholar]
  15. Hoffmann P. J. Mechanism of degradation of duplex DNA by the DNase induced by herpes simplex virus. J Virol. 1981 Jun;38(3):1005–1014. doi: 10.1128/jvi.38.3.1005-1014.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knopf K. W. Properties of herpes simplex virus DNA polymerase and characterization of its associated exonuclease activity. Eur J Biochem. 1979 Jul;98(1):231–244. doi: 10.1111/j.1432-1033.1979.tb13181.x. [DOI] [PubMed] [Google Scholar]
  17. Lin J. C., Shaw J. E., Smith M. C., Pagano J. S. Effect of 12-O-tetradecanoyl-phorbol-13-acetate on the replication of Epstein-Barr virus. I. Characterization of viral DNA. Virology. 1979 Nov;99(1):183–187. doi: 10.1016/0042-6822(79)90052-7. [DOI] [PubMed] [Google Scholar]
  18. Miller R. L., Glaser R., Rapp F. Studies of an Epstein-Barr virus-induced DNA polymerase. Virology. 1977 Feb;76(2):494–502. doi: 10.1016/0042-6822(77)90232-x. [DOI] [PubMed] [Google Scholar]
  19. Ooka T., Lenoir G., Daillie J. Characterization of an Epstein-Barr virus-induced DNA polymerase. J Virol. 1979 Jan;29(1):1–10. doi: 10.1128/jvi.29.1.1-10.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ostrander M., Cheng Y. C. Properties of herpes simplex virus type 1 and type 2 DNA polymerase. Biochim Biophys Acta. 1980 Sep 19;609(2):232–245. doi: 10.1016/0005-2787(80)90234-8. [DOI] [PubMed] [Google Scholar]
  21. Paul W. E., Sredni B., Schwartz R. H. Long-term growth and cloning of non-transformed lymphocytes. Nature. 1981 Dec 24;294(5843):697–699. doi: 10.1038/294697a0. [DOI] [PubMed] [Google Scholar]
  22. Potuzak H., Wintersberger U. DNA covalently linked to carboxymethyl-cellulose and its application in affinity chromatography. FEBS Lett. 1976 Mar 15;63(1):167–170. doi: 10.1016/0014-5793(76)80218-9. [DOI] [PubMed] [Google Scholar]
  23. Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Strobel-Fidler M., Francke B. Alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme. Virology. 1980 Jun;103(2):493–501. doi: 10.1016/0042-6822(80)90206-8. [DOI] [PubMed] [Google Scholar]
  25. Weissbach A., Hong S. C., Aucker J., Muller R. Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase. J Biol Chem. 1973 Sep 25;248(18):6270–6277. [PubMed] [Google Scholar]
  26. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  27. zur Hausen H., Bornkamm G. W., Schmidt R., Hecker E. Tumor initiators and promoters in the induction of Epstein-Barr virus. Proc Natl Acad Sci U S A. 1979 Feb;76(2):782–785. doi: 10.1073/pnas.76.2.782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. zur Hausen H., O'Neill F. J., Freese U. K., Hecker E. Persisting oncogenic herpesvirus induced by the tumour promotor TPA. Nature. 1978 Mar 23;272(5651):373–375. doi: 10.1038/272373a0. [DOI] [PubMed] [Google Scholar]

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