Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1982 Dec;44(3):983–992. doi: 10.1128/jvi.44.3.983-992.1982

Association of viral particles and viral proteins with membranes in SA11-infected cells.

C Soler, C Musalem, M Loroño, R T Espejo
PMCID: PMC256358  PMID: 6294346

Abstract

Electron microscopy after negative staining of SA11-infected cell homogenates revealed that most of the viral particles are associated with membrane-like material. Many of the particles seemed to be fully enveloped in a membrane. This association could also be detected by the observed cosedimentation of viral proteins and cell membranes. Pulse-chase experiments showed that viral glycoproteins rapidly associate with membranes, whereas most of the structural proteins appearing in the soluble fraction immediately after the pulse were slowly chased into the membrane fraction. The membranes could be further fractionated into at least four fractions differing in density and containing a different distribution of viral proteins. Also, the distribution of label into each of these membrane fractions changed after long chase periods. The inhibition of glycosylation with tunicamycin yielded viral particles without an outer layer, but did not affect the described association with membranes. The possible relationship of this finding to the maturation of the virion is discussed.

Full text

PDF
983

Images in this article

985Image
on p.985
987Image
on p.987
989Image
on p.989
990Image
on p.990

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams W. R., Kraft L. M. Electron-Microscopic Study of the Intestinal Epithelium of Mice Infected with the Agent of Epizootic Diarrhea of Infant Mice (EDIM Virus). Am J Pathol. 1967 Jul;51(1):39–60. [PMC free article] [PubMed] [Google Scholar]
  2. Altenburg B. C., Graham D. Y., Estes M. K. Ultrastructural study of rotavirus replication in cultured cells. J Gen Virol. 1980 Jan;46(1):75–85. doi: 10.1099/0022-1317-46-1-75. [DOI] [PubMed] [Google Scholar]
  3. Arias C. F., López S., Espejo R. T. Gene protein products of SA11 simian rotavirus genome. J Virol. 1982 Jan;41(1):42–50. doi: 10.1128/jvi.41.1.42-50.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Carpio M. M., Babiuk L. A., Misra V., Blumenthal R. M. Bovine rotavirus-cell interactions: effect of virus infection on cellular integrity and macromolecular synthesis. Virology. 1981 Oct 15;114(1):86–97. doi: 10.1016/0042-6822(81)90255-5. [DOI] [PubMed] [Google Scholar]
  6. Chasey D. Investigation of immunoperoxidase-labelled rotavirus in tissue culture by light and electron microscopy. J Gen Virol. 1980 Sep;50(1):195–200. doi: 10.1099/0022-1317-50-1-195. [DOI] [PubMed] [Google Scholar]
  7. Dyall-Smith M. L., Holmes I. H. Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. J Virol. 1981 Dec;40(3):720–728. doi: 10.1128/jvi.40.3.720-728.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Els H. J., Lecatsas G. Morphological studies on simian virus S.A. 11 and the 'related' O agent. J Gen Virol. 1972 Oct;17(1):129–132. doi: 10.1099/0022-1317-17-1-129. [DOI] [PubMed] [Google Scholar]
  9. Els H. J., Verwoerd D. W. Morphology of bluetongue virus. Virology. 1969 Jun;38(2):213–219. doi: 10.1016/0042-6822(69)90362-6. [DOI] [PubMed] [Google Scholar]
  10. Erwin C., Brown D. T. Intracellular distribution of Sindbis virus membrane proteins in BHK-21 cells infected with wild-type virus and maturation-defective mutants. J Virol. 1980 Dec;36(3):775–786. doi: 10.1128/jvi.36.3.775-786.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Espejo R. T., López S., Arias C. Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. J Virol. 1981 Jan;37(1):156–160. doi: 10.1128/jvi.37.1.156-160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Estes M. K., Graham D. Y., Smith E. M., Gerba C. P. Rotavirus stability and inactivation. J Gen Virol. 1979 May;43(2):403–409. doi: 10.1099/0022-1317-43-2-403. [DOI] [PubMed] [Google Scholar]
  13. Flewett T. H., Woode G. N. The rotaviruses. Arch Virol. 1978;57(1):1–23. doi: 10.1007/BF01315633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holmes I. H. Viral gastroenteritis. Prog Med Virol. 1979;25:1–36. [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lecatsas G. Electron microscopic and serological studies on simian virus S.A. 11 and the "related" O agent. Onderstepoort J Vet Res. 1972 Sep;39(3):133–137. [PubMed] [Google Scholar]
  17. Malherbe H. H., Strickland-Cholmley M. Simian virus SA11 and the related O agent. Arch Gesamte Virusforsch. 1967;22(1):235–245. doi: 10.1007/BF01240518. [DOI] [PubMed] [Google Scholar]
  18. McNulty M. S., Curran W. L., McFerran J. B. The morphogenesis of a cytopathic bovine rotavirus in Madin-Darby bovine kidney cells. J Gen Virol. 1976 Dec;33(3):503–508. doi: 10.1099/0022-1317-33-3-503. [DOI] [PubMed] [Google Scholar]
  19. Petrie B. L., Graham D. Y., Estes M. K. Identification of rotavirus particle types. Intervirology. 1981;16(1):20–28. doi: 10.1159/000149243. [DOI] [PubMed] [Google Scholar]
  20. Rodger S. M., Holmes I. H. Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol. 1979 Jun;30(3):839–846. doi: 10.1128/jvi.30.3.839-846.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Saif L. J., Theil K. W., Bohl E. H. Morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells. J Gen Virol. 1978 May;39(2):205–217. doi: 10.1099/0022-1317-39-2-205. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES