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. 1982 Dec;44(3):1039–1046. doi: 10.1128/jvi.44.3.1039-1046.1982

Protease bypass of temperature-sensitive murine leukemia virus maturation mutants.

P Traktman, D Baltimore
PMCID: PMC256364  PMID: 6184485

Abstract

Cells infected with certain temperature-sensitive mutants of Moloney murine leukemia virus synthesize the virion precursor proteins but neither bud virions nor cleave precursor proteins to their mature form. Addition of proteases to cells infected with these mutants caused cleavage of the precursor proteins Pr65gag and Pr180gag-pol to their mature forms at the nonpermissive temperature. Concomitantly there was release from the cells of morphologically normal virions. The enzymatically inactive Pr180gag-pol was cleaved to active reverse transcriptase (p85), which was found in the released particles. External protease treatment apparently bypassed the lesion in these viral mutants, suggesting that their defect may involve a virus-specific protease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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