Abstract
Tryptic peptides containing two major in vivo P120gag-abl tyrosine phosphorylation acceptor sites were identified, phosphorylated in vitro, and purified to homogeneity. The tyrosine site in peptide a is localized at a position six residues distal to its trypsin cleavage site, whereas the tyrosine acceptor site in peptide b is at residue seven. A third peptide, c, contains an amino-terminal phosphotyrosine residue: phosphorylation of this latter peptide only occurs to a significant extent in vivo.
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- Abelson H. T., Rabstein L. S. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 1970 Aug;30(8):2213–2222. [PubMed] [Google Scholar]
- Blomberg J., Reynolds F. H., Jr, Van de Ven W. J., Stephenson J. R. Abelson murine leukaemia virus transformation involves loss of epidermal growth factor-binding sites. Nature. 1980 Jul 31;286(5772):504–507. doi: 10.1038/286504a0. [DOI] [PubMed] [Google Scholar]
- Blomberg J., Van de Ven W. J., Reynolds F. H., Jr, Nalewaik R. P., Stephenson J. R. Snyder-Theilen feline sarcoma virus P85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase. J Virol. 1981 Jun;38(3):886–894. doi: 10.1128/jvi.38.3.886-894.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Copeland T. D., Grandgenett D. P., Oroszlan S. Amino acid sequence analysis of reverse transcriptase subunits from avian myeloblastosis virus. J Virol. 1980 Oct;36(1):115–119. doi: 10.1128/jvi.36.1.115-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neil J. C., Ghysdael J., Vogt P. K., Smart J. E. Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses. Nature. 1981 Jun 25;291(5817):675–677. doi: 10.1038/291675a0. [DOI] [PubMed] [Google Scholar]
- Patschinsky T., Hunter T., Esch F. S., Cooper J. A., Sefton B. M. Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation. Proc Natl Acad Sci U S A. 1982 Feb;79(4):973–977. doi: 10.1073/pnas.79.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynolds F. H., Jr, Sacks T. L., Deobagkar D. N., Stephenson J. R. Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3974–3978. doi: 10.1073/pnas.75.8.3974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynolds F. H., Jr, Van de Ven W. J., Stephenson J. R. Abelson murine leukemia virus transformation-defective mutants with impaired P120-associated protein kinase activity. J Virol. 1980 Nov;36(2):374–386. doi: 10.1128/jvi.36.2.374-386.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynolds R. K., van de Ven W. J., Stephenson J. R. Translation of type C viral RNAs in Xenopus laevis oocytes: evidence that the 120,000-molecular-weight polyprotein expressed in Abelson leukemia virus-transformed cells is virus coded. J Virol. 1978 Nov;28(2):665–670. doi: 10.1128/jvi.28.2.665-670.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg N., Baltimore D., Scher C. D. In vitro transformation of lymphoid cells by Abelson murine leukemia virus. Proc Natl Acad Sci U S A. 1975 May;72(5):1932–1936. doi: 10.1073/pnas.72.5.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scher C. D., Siegler R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature. 1975 Feb 27;253(5494):729–731. doi: 10.1038/253729a0. [DOI] [PubMed] [Google Scholar]
- Sefton B. M., Hunter T., Raschke W. C. Evidence that the Abelson virus protein functions in vivo as a protein kinase that phosphorylates tyrosine. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1552–1556. doi: 10.1073/pnas.78.3.1552. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smart J. E., Oppermann H., Czernilofsky A. P., Purchio A. F., Erikson R. L., Bishop J. M. Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src). Proc Natl Acad Sci U S A. 1981 Oct;78(10):6013–6017. doi: 10.1073/pnas.78.10.6013. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Twardzik D. R., Todaro G. J., Marquardt H., Reynolds F. H., Jr, Stephenson J. R. Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor. Science. 1982 May 21;216(4548):894–897. doi: 10.1126/science.6177040. [DOI] [PubMed] [Google Scholar]
- Van de Ven W. J., Reynolds F. H., Jr, Nalewaik R. P., Stephenson J. R. The nonstructural component of the Abelson murine leukemia virus polyprotein P120 is encoded by newly acquired genetic sequences. J Virol. 1979 Dec;32(3):1041–1045. doi: 10.1128/jvi.32.3.1041-1045.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van de Ven W. J., Reynolds F. H., Jr, Stephenson J. R. The nonstructural components of polyproteins encoded by replication-defective mammalian transforming retroviruses are phosphorylated and have associated protein kinase activity. Virology. 1980 Feb;101(1):185–197. doi: 10.1016/0042-6822(80)90495-x. [DOI] [PubMed] [Google Scholar]
- Witte O. N., Dasgupta A., Baltimore D. Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine. Nature. 1980 Feb 28;283(5750):826–831. doi: 10.1038/283826a0. [DOI] [PubMed] [Google Scholar]
- Witte O. N., Goff S., Rosenberg N., Baltimore D. A transformation-defective mutant of Abelson murine leukemia virus lacks protein kinase activity. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4993–4997. doi: 10.1073/pnas.77.8.4993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witte O. N., Ponticelli A., Gifford A., Baltimore D., Rosenberg N., Elder J. Phosphorylation of the Abelson murine leukemia virus transforming protein. J Virol. 1981 Sep;39(3):870–878. doi: 10.1128/jvi.39.3.870-878.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witte O. N., Rosenberg N., Paskind M., Shields A., Baltimore D. Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2488–2492. doi: 10.1073/pnas.75.5.2488. [DOI] [PMC free article] [PubMed] [Google Scholar]


