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. 1983 Jan;45(1):428–433. doi: 10.1128/jvi.45.1.428-433.1983

Subcellular localization of the simian virus 40 agnoprotein.

S Nomura, G Khoury, G Jay
PMCID: PMC256424  PMID: 6296448

Abstract

The intracellular distribution of the simian virus 40 agnoprotein in infected cells was determined by indirect immunofluorescence and biochemical fractionation followed by indirect immunoprecipitation. The specific antibodies used in these studies were directed against either purified agnoprotein or a synthetic oligopeptide homologous to the N-terminus of the processed protein. Both procedures showed predominant localization of the agnoprotein to the cytosol and to the perinuclear region in association with the outer nuclear membrane. A minor but detectable fraction of the protein was also found in the nucleus. The definition of its subcellular distribution, as well as its high lability in vivo and affinity for nucleic acid, provide a basis for speculation on the function of this gene product.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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