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. 1983 Feb;45(2):585–593. doi: 10.1128/jvi.45.2.585-593.1983

Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis.

B B Gourlie, V P Pigiet
PMCID: PMC256452  PMID: 6300422

Abstract

A two-dimensional (neutral-alkali) agarose gel electrophoretic system was used to separate three families of replicative intermediate (RI) polyoma virus DNA molecules (form I, form II, and form III RIs). Two of these families, form II and III RIs, are the result of artifactual nicking of one of the parental strands of supercoiled RIs (form I RIs) during in vitro replication of soluble minichromosomes. Kinetic studies in vitro showed that the nicked RIs serve as templates for limited DNA synthesis. The nicked species are not converted into normal products, however. The nicking reaction, which appears to be specific for the parental strands, is dependent on magnesium ions and occurs concurrently with the in vitro synthesis of DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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