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. 1983 Feb;45(2):773–781. doi: 10.1128/jvi.45.2.773-781.1983

Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein.

J Sprague, J H Condra, H Arnheiter, R A Lazzarini
PMCID: PMC256472  PMID: 6300434

Abstract

A cDNA clone containing the entire vesicular stomatitis virus nucleocapsid gene was assembled by fusing portions of two partial clones. When the cDNA clone was inserted into a new general-purpose eucaryotic expression vector and introduced into appropriate host cells, abundant N-protein synthesis ensued. The expressed protein was indistinguishable from authentic N protein produced during vesicular stomatitis virus infections. The recombinant N protein was recognized by a polyclonal antibody and two different monoclonal antibodies and could not be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis from authentic N. Our results suggest that the recombinant N protein produced in transfected cells rapidly aggregates into high-molecular-weight complexes in the absence of vesicular stomatitis virus genomic RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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