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. 1983 Feb;45(2):882–884. doi: 10.1128/jvi.45.2.882-884.1983

Isolation of the Ends of La Crosse Virus Small RNA as a Double-Stranded Structure

Jean L Patterson 1, Daniel Kolakofsky 1, Brian P Holloway 2, John F Obijeski 2,†
PMCID: PMC256485  PMID: 6834474

Abstract

The genome of La Crosse virus, a member of the Bunyaviridae, is made up of three molecules. Circular nucleocapsid structures, in three size classes, have been isolated from La Crosse virus (Obijeski et al. J. Virol. 20:664-675, 1976). Recently, Obijeski et al. (Nucleic Acids Res. 8:2431-2438) have found that the 5′ and 3′ ends of each segment are complementary in sequence. We determined that a 5′ and 3′ end complementary structure, predicted by the rules of Tinoco et al. (Nature [London] 230:362-367), can and will anneal under certain conditions. This structure is resistant to RNase in high-salt medium and can be isolated in a reasonably high yield.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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