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. Author manuscript; available in PMC: 2008 Oct 8.
Published in final edited form as: Biochemistry. 2006 Jan 24;45(3):976–986. doi: 10.1021/bi051978h

Figure 2. Photoincorporation of [3H]Azicholesterol into nAChR-enriched membranes in the absence and presence of carbamylcholine.

Figure 2

nAChR-enriched membranes were equilibrated (2 h incubation) with 1.25 μM [3H]Azicholesterol in the absence (− lanes) and in the presence (+ lanes) of 400μM carbamylcholine and irradiated at 365 nm for 10 min. After irradiation, polypeptides were resolved by SDS-PAGE and processed for fluorography (3-week exposure). Labeled lipid and free photolysis products were electrophoresed from the gel with the tracking dye. Indicated on the left are the mobility of nAChR subunits (α,β,γ,δ), the α-subunit of Na+/K+-ATPase (αNK, ∼95kDa), and the mitochondrial voltage-dependant anion channel, VDAC (∼34 kDa).