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. Author manuscript; available in PMC: 2008 Oct 8.
Published in final edited form as: Biochemistry. 2006 Jan 24;45(3):976–986. doi: 10.1021/bi051978h

Figure 9. Reversed-phase HPLC purification of [3H]Azicholesterol labeled fragments from tryptic digests of αV8-20, βV8-22, γV8-24, and δV8-20.

Figure 9

The [3H]Azicholesterol labeled tryptic peptides (∼ 4 kDa) isolated from exhaustive digests of αV8-20 (A), βV8-22 (B), γV8-24 (C), and δV8-20 (D) that were resolved by Tricine SDS-PAGE were further purified by reversed-phase HPLC as described in the Experimental Procedures and in the legend for Fig.5. The elution of peptides was monitored by absorbance at 210 nm (solid line) and elution of 3H by scintillation counting of aliquots (50 μL) of each 500 μL fraction (●). The point of elution of the M1 peptide based on previous work (and from sequencing results from this report) is indicated with an arrow. Based upon recovery of radioactivity, >90% of the material was recovered from the HPLC column.