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. 1983 Mar;45(3):956–960. doi: 10.1128/jvi.45.3.956-960.1983

Mouse immunoglobulin antibodies require complement for neutralization of mouse retroviruses.

P S Sarma, W P Rowe
PMCID: PMC256501  PMID: 6300466

Abstract

The addition of guinea pig complement was found to enhance the neutralizing capacity of mouse antibodies directed against the endogenous ecotropic murine leukemia viruses. The same immune sera, when tested without complement, had low or negligible neutralizing capacities, regardless of whether freshly harvested, unfrozen virus was used to preserve virus infectivity. Antibodies in high titers were found in sera from NFS congenic mice carrying the mouse leukemia virus inducing locus Akv-2. These mouse antibodies were type specific and failed to neutralize either Friend or Moloney leukemia virus. The mouse serum immunoglobulin fraction containing the complement-dependent antibodies was tentatively identified as immunoglobulin M.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Stephenson J. R. Widespread natural occurrence of high titers of neutralizing antibodies to a specific class of endogenous mouse type-C virus. Proc Natl Acad Sci U S A. 1974 May;71(5):1957–1961. doi: 10.1073/pnas.71.5.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adler F. L., Walker W. S., Fishman M. Amplification of phage neutralization by complement, antiglobulin, and antiallotype sera. Virology. 1971 Dec;46(3):797–807. doi: 10.1016/0042-6822(71)90081-x. [DOI] [PubMed] [Google Scholar]
  3. Almeida J. D., Waterson A. P. The morphology of virus-antibody interaction. Adv Virus Res. 1969;15:307–338. doi: 10.1016/S0065-3527(08)60878-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cloyd M. W., Hartley J. W., Rowe W. P. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980 Mar 1;151(3):542–552. doi: 10.1084/jem.151.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Daniels C. A., Borsos T., Rapp H. J., Snyderman R., Notkins A. L. Neutralization of sensitized virus by purified components of complement. Proc Natl Acad Sci U S A. 1970 Mar;65(3):528–535. doi: 10.1073/pnas.65.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham B. J., Minamishima Y., Dresman G. R., Haines H. G., Benyesh-Melnick M. Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses. J Immunol. 1971 Dec;107(6):1618–1630. [PubMed] [Google Scholar]
  7. Hampar B., Notkins A. L., Mage M., Keehn M. A. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus. J Immunol. 1968 Mar;100(3):586–593. [PubMed] [Google Scholar]
  8. Hanna M. G., Jr, Ihle J. N., Batzing B. L., Tennant T. W., Schenley C. K. Assessment of reactivities of natural antibodies to endogenous RNA tumor virus envelope antigens and virus-induced cell surface antigens. Cancer Res. 1975 Jan;35(1):164–171. [PubMed] [Google Scholar]
  9. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  10. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heineman H. S. Herpes simplex neutralizing antibody--quantitation of the complement-dependent fraction in different phases of adult human infection. J Immunol. 1967 Jul;99(1):214–222. [PubMed] [Google Scholar]
  12. Ihle J. N., Denny T. P., Bolognesi D. P. Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice. J Virol. 1976 Mar;17(3):727–736. doi: 10.1128/jvi.17.3.727-736.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ihle J. N., Lazar B. Natural immunity in mice to the envelope glycoprotein of endogenous ecotropic type C viruses: neutralization of virus infectivity. J Virol. 1977 Mar;21(3):974–980. doi: 10.1128/jvi.21.3.974-980.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ishizaki R., Vogt P. K. Immunological relationships among envelope antigens of avian tumor viruses. Virology. 1966 Nov;30(3):375–387. doi: 10.1016/0042-6822(66)90116-4. [DOI] [PubMed] [Google Scholar]
  15. Iwasaki T., Ogura R. Studies on complement-potentiated neutralizing antibodies (C'-PNAb) induced in rabbits inoculated with japanese encephalitis virus (JEV). I. The nature of C'-PNAb. Virology. 1968 Jan;34(1):46–59. doi: 10.1016/0042-6822(68)90007-x. [DOI] [PubMed] [Google Scholar]
  16. Lee J. C., Hanna M. G., Jr, Ihle J. N., Aaronson S. A. Autogenous immunity to endogenous RNA tumor virus: differential reactivities of immunoglobulins M and G to virus envelope antigens. J Virol. 1974 Oct;14(4):773–781. doi: 10.1128/jvi.14.4.773-781.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Levy J. A., Ihle J. N., Oleszko O., Barnes R. D. Virus-specific neutralization by a soluble non-immunoglobulin factor found naturally in normal mouse sera. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5071–5075. doi: 10.1073/pnas.72.12.5071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Linscott W. D., Levinson W. E. Complement components required for virus neutralization by early immunoglobulin antibody. Proc Natl Acad Sci U S A. 1969 Oct;64(2):520–527. doi: 10.1073/pnas.64.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Martin S. E., Martin W. J. Naturally occurring cytotoxic tumour reactive antibodies directed against type C viral envelope antigens. Nature. 1975 Aug 7;256(5517):498–499. doi: 10.1038/256498a0. [DOI] [PubMed] [Google Scholar]
  20. Nowinski R. C., Kaehler S. L. Antibody to leukemia virus: widespread occurrence in inbred mice. Science. 1974 Sep 6;185(4154):869–871. doi: 10.1126/science.185.4154.869. [DOI] [PubMed] [Google Scholar]
  21. Oldstone M. B. Virus neutralization and virus-induced immune complex disease. Virus-antibody union resulting in immunoprotection or immunologic injury--two sides of the same coin. Prog Med Virol. 1975;19:84–119. [PubMed] [Google Scholar]
  22. Oroszlan S., Gilden R. V. Immune virolysis: effect of antibody and complement on C-type RNA virus. Science. 1970 Jun 19;168(3938):1478–1480. doi: 10.1126/science.168.3938.1478. [DOI] [PubMed] [Google Scholar]
  23. Rowe W. P., Hartley J. W., Bremner T. Genetic mapping of a murine leukemia virus-inducing locus of AKR mice. Science. 1972 Nov 24;178(4063):860–862. doi: 10.1126/science.178.4063.860. [DOI] [PubMed] [Google Scholar]
  24. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  25. Rowe W. P. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice. J Exp Med. 1972 Nov 1;136(5):1272–1285. doi: 10.1084/jem.136.5.1272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SARMA P. S., HUEBNER R. J., ARMSTRONG D. A SIMPLIFIED TISSUE CULTURE TUBE NEUTRALIZATION TEST FOR ROUS SARCOMA VIRUS ANTIBODIES. Proc Soc Exp Biol Med. 1964 Feb;115:481–486. doi: 10.3181/00379727-115-28947. [DOI] [PubMed] [Google Scholar]
  27. Sarma P. S., Sharar A., Walters V., Gardner M. A survey of cats and humans for prevalence of feline leukemia-sarcoma virus neutralizing serum antibodies. Proc Soc Exp Biol Med. 1974 Feb;145(2):560–564. doi: 10.3181/00379727-145-37851. [DOI] [PubMed] [Google Scholar]
  28. Sherwin S. A., Benveniste R. E., Todaro G. J. Complement-mediated lysis of type-C virus: effect of primate and human sera on various retroviruses. Int J Cancer. 1978 Jan 15;21(1):6–11. doi: 10.1002/ijc.2910210103. [DOI] [PubMed] [Google Scholar]
  29. Wallis C., Melnick J. L. Herpesvirus neutralization: the role of complement. J Immunol. 1971 Nov;107(5):1235–1242. [PubMed] [Google Scholar]
  30. Welsh R. M., Jr, Jensen F. C., Cooper N. R., Oldstone M. B. Inactivation of lysis of oncornaviruses by human serum. Virology. 1976 Oct 15;74(2):432–440. doi: 10.1016/0042-6822(76)90349-4. [DOI] [PubMed] [Google Scholar]

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