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. 1983 Mar;45(3):1211–1216. doi: 10.1128/jvi.45.3.1211-1216.1983

Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.

D Bryant, J T Parsons
PMCID: PMC256536  PMID: 6300458

Abstract

Site-directed mutagenesis techniques were used to construct defined point mutations within the src gene of the Prague A strain of Rous sarcoma virus. Bisulfite mutagenesis at a Bg/I restriction site in the src gene yielded three mutations which contained the same single base change, a guanine-to-adenine transition. The resulting genomes encoded an src protein containing a substitution of threonine for alanine at amino acid position 433. Transfection of chicken cells with mutagenized DNA did not result in cellular transformation even though the cells produced a pp60src. Immune complexes containing mutant pp60src did not phosphorylate immunoglobulin G heavy chain or casein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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