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. 1983 Jun;46(3):709–717. doi: 10.1128/jvi.46.3.709-717.1983

Conditional synthesis of an aberrant glycoprotein mRNA by the internal deletion mutant of vesicular stomatitis virus.

R C Herman
PMCID: PMC256547  PMID: 6133960

Abstract

The internal deletion mutant (DI-LT) derived from the heat-resistant strain of vesicular stomatitis virus synthesizes an aberrant polyadenylated mRNA (G*) containing a transcript of the partially deleted polymerase gene covalently linked to the 3' end of the glycoprotein message (R. C. Herman and R. A. Lazzarini, J. Virol. 40:78-86, 1981). The heat-resistant polymerase appears to play a role in the synthesis of the abnormal G* RNA. The synthesis of G* correlated directly with the presence of the heat-resistant L protein on the defective interfering particle template. Chimeric defective interfering particles produced by passaging DI-LT with a helper virus that encodes the wild-type vesicular stomatitis virus polymerase did not synthesize G*. The subsequent passage of the chimeric DI-LT with a heat-resistant helper virus restored the ability to synthesize the G* transcript. These results imply that the regulatory signals normally present at the vesicular stomatitis virus G/L intercistronic boundary may be preserved in DI-LT. These sequences are only conditionally functional because they are recognized correctly by the wild-type but not by the heat-resistant polymerase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball L. A. Transcriptional mapping of vesicular stomatitis virus in vivo. J Virol. 1977 Jan;21(1):411–414. doi: 10.1128/jvi.21.1.411-414.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball L. A., White C. N. Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):442–446. doi: 10.1073/pnas.73.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Banerjee A. D., Abraham G., Colonno R. J. Vesicular stomatitis virus: mode of transcription. J Gen Virol. 1977 Jan;34(1):1–8. doi: 10.1099/0022-1317-34-1-1. [DOI] [PubMed] [Google Scholar]
  5. Bay P. H., Reichmann M. E. In vitro and in vivo inhibition of primary transcription of vesicular stomatitis virus by a defective interfering particle. J Virol. 1982 Jan;41(1):172–182. doi: 10.1128/jvi.41.1.172-182.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bay P. H., Reichmann M. E. UV inactivation of the biological activity of defective interfering particles generated by vesicular stomatitis virus. J Virol. 1979 Dec;32(3):876–884. doi: 10.1128/jvi.32.3.876-884.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chanda P. K., Kang C. Y., Banerjee A. K. Synthesis in vitro of the full-length complement of defective-interfering particle RNA of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3927–3931. doi: 10.1073/pnas.77.7.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clerx-Van Haaster C. M., Clewley J. P., Bishop D. H. Oligonucleotide sequence analyses indicate that vesicular stomatitis virus large defective interfering virus particle RNA is made by internal deletion: evidence for similar transcription polyadenylation signals for the synthesis of all vesicular stomatitis virus mRNA species. J Virol. 1980 Feb;33(2):807–817. doi: 10.1128/jvi.33.2.807-817.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Colonno R. J., Lazzarini R. A., Keene J. D., Banerjee A. K. In vitro synthesis of messenger RNA by a defective interfering particle of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1977 May;74(5):1884–1888. doi: 10.1073/pnas.74.5.1884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Emerson S. U., Yu Y. Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1975 Jun;15(6):1348–1356. doi: 10.1128/jvi.15.6.1348-1356.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Epstein D. A., Herman R. C., Chien I., Lazzarini R. A. Defective interfering particle generated by internal deletion of the vesicular stomatitis virus genome. J Virol. 1980 Feb;33(2):818–829. doi: 10.1128/jvi.33.2.818-829.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herman R. C., Adler S., Lazzarini R. A., Colonno R. J., Banerjee A. K., Westphal H. Intervening polyadenylate sequences in RNA transcripts of vesicular stomatitis virus. Cell. 1978 Oct;15(2):587–596. doi: 10.1016/0092-8674(78)90027-2. [DOI] [PubMed] [Google Scholar]
  13. Herman R. C., Lazzarini R. A. Aberrant glycoprotein mRNA synthesized by the internal deletion mutant of vesicular stomatitis virus. J Virol. 1981 Oct;40(1):78–86. doi: 10.1128/jvi.40.1.78-86.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herman R. C., Lazzarini R. A. Vesicular stomatitis virus RNA polymerase can read through the boundary between the leader and N genes in vitro. J Virol. 1981 May;38(2):792–796. doi: 10.1128/jvi.38.2.792-796.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Herman R. C., Schubert M., Keene J. D., Lazzarini R. A. Polycistronic vesicular stomatitis virus RNA transcripts. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4662–4665. doi: 10.1073/pnas.77.8.4662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson L. D., Binder M., Lazzarini R. A. A defective interfering vesicular stomatitis virus particle that directs the synthesis of functional proteins in the absence of helper virus. Virology. 1979 Nov;99(1):203–206. doi: 10.1016/0042-6822(79)90056-4. [DOI] [PubMed] [Google Scholar]
  17. Johnson L. D., Lazzarini R. A. Replication of viral RNA by a defective interfering vesicular stomatitis virus particle in the absence of helper virus. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4387–4391. doi: 10.1073/pnas.74.10.4387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Keene J. D., Chien I. M., Lazzarini R. A. Vesicular stomatitis virus defective interfering particle containing a muted internal leader RNA gene. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2090–2094. doi: 10.1073/pnas.78.4.2090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leamnson R. N., Reichmann M. E. The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons. J Mol Biol. 1974 Jan 5;85(4):551–568. doi: 10.1016/0022-2836(74)90315-5. [DOI] [PubMed] [Google Scholar]
  20. McGeoch D. J. Structure of the gene N:gene NS intercistronic junction in the genome of vesicular stomatitis virus. Cell. 1979 Jul;17(3):673–681. doi: 10.1016/0092-8674(79)90274-5. [DOI] [PubMed] [Google Scholar]
  21. Moyer S. A., Banerjee A. K. Messenger RNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus. Cell. 1975 Jan;4(1):37–43. doi: 10.1016/0092-8674(75)90131-2. [DOI] [PubMed] [Google Scholar]
  22. Moyer S. A., Grubman M. J., Ehrenfeld E., Banerjee A. K. Studies on the in vivo and in vitro messenger RNA species of vesicular stomatitis virus. Virology. 1975 Oct;67(2):463–473. doi: 10.1016/0042-6822(75)90447-x. [DOI] [PubMed] [Google Scholar]
  23. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  24. Perrault J., Semler B. L. Internal genome deletions in two distinct classes of defective interfering particles of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6191–6195. doi: 10.1073/pnas.76.12.6191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Petric M., Prevec L. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970 Aug;41(4):615–630. doi: 10.1016/0042-6822(70)90427-7. [DOI] [PubMed] [Google Scholar]
  26. Rose J. K. Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell. 1980 Feb;19(2):415–421. doi: 10.1016/0092-8674(80)90515-2. [DOI] [PubMed] [Google Scholar]
  27. Rose J. K. Heterogneeous 5'-terminal structures occur on vesicular stomatitis virus mRNAs. J Biol Chem. 1975 Oct 25;250(20):8098–8104. [PubMed] [Google Scholar]
  28. Schnitzlein W. M., Reichmann M. E. The size and the cistronic origin of defective vesicular stomatitis virus particle RNAs in relation to homotypic and heterotypic interference. J Mol Biol. 1976 Mar 5;101(3):307–325. doi: 10.1016/0022-2836(76)90150-9. [DOI] [PubMed] [Google Scholar]
  29. Stamminger G., Lazzarini R. A. Analysis of the RNA of defective VSV particles. Cell. 1974 Sep;3(1):85–93. doi: 10.1016/0092-8674(74)90044-0. [DOI] [PubMed] [Google Scholar]
  30. Testa D., Banerjee A. K. In vitro synthesis of a possible precursor RNA by purified vesicular stomatitis virus. Biochem Biophys Res Commun. 1978 May 30;82(2):655–664. doi: 10.1016/0006-291x(78)90925-7. [DOI] [PubMed] [Google Scholar]
  31. Testa D., Chanda P. K., Banerjee A. K. In vitro synthesis of the full-length complement of the negative-strand genome RNA of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1980 Jan;77(1):294–298. doi: 10.1073/pnas.77.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Testa D., Chanda P. K., Banerjee A. K. Unique mode of transcription in vitro by Vesicular stomatitis virus. Cell. 1980 Aug;21(1):267–275. doi: 10.1016/0092-8674(80)90134-8. [DOI] [PubMed] [Google Scholar]
  33. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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