Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1981 Oct;40(1):118–125. doi: 10.1128/jvi.40.1.118-125.1981

Restriction map of the single-stranded DNA genome of Kilham rat virus strains 171, a nondefective parvovirus.

P T Banerjee, R Rothrock, S Mitra
PMCID: PMC256601  PMID: 6270368

Abstract

We constructed a physical map of Kilham rat virus strains 171 DNA by analyzing the sizes and locations of restriction endonuclease-generated fragments of the replicative-form viral DNA synthesized in vitro. BglI, KpnI, BamHI, SmaI, XhoI, and XorII did not appear to have any cleavage sites, whereas 11 other enzymes cleaved the genome at one to eight sites, and AluI generated more than 12 distinct fragments. The 30 restriction sites that were mapped were distributed randomly in the viral genome. A comparison of the restriction fragments of in vivo- and in vitro-replicated replicative-form DNAs showed that these DNAs were identical except in the size or configuration of the terminal fragments.

Full text

PDF

Images in this article

122Image
on p.122
123Image
on p.123
124Image
on p.124

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astell C. R., Smith M., Chow M. B., Ward D. C. Sequence of the 3' terminus of the genome from Kilham rat virus, a nondefective parvovirus. Virology. 1979 Jul 30;96(2):669–674. doi: 10.1016/0042-6822(79)90127-2. [DOI] [PubMed] [Google Scholar]
  2. Astell C. R., Smith M., Chow M. B., Ward D. C. Structure of the 3' hairpin termini of four rodent parvovirus genomes: nucleotide sequence homology at origins of DNA replication. Cell. 1979 Jul;17(3):691–703. doi: 10.1016/0092-8674(79)90276-9. [DOI] [PubMed] [Google Scholar]
  3. Berns K. I., Hauswirth W. W. Adeno-associated viruses. Adv Virus Res. 1979;25:407–449. doi: 10.1016/s0065-3527(08)60574-6. [DOI] [PubMed] [Google Scholar]
  4. Bourguignon G. J., Tattersall P. J., Ward D. C. DNA of minute virus of mice: self-priming, nonpermuted, single-stranded genome with a 5'-terminal hairpin duplex. J Virol. 1976 Oct;20(1):290–306. doi: 10.1128/jvi.20.1.290-306.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Das S. K., Fujimura R. K. Exonuclease associated with bacteriophage T5-Induced DNA polymerase. J Virol. 1976 Oct;20(1):70–77. doi: 10.1128/jvi.20.1.70-77.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Faust E. A., Ward D. C. Incomplete genomes of the parvovirus minute virus of mice: selective conservation of genome termini, including the origin for DNA replication. J Virol. 1979 Oct;32(1):276–292. doi: 10.1128/jvi.32.1.276-292.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujimura R. K., Roop B. C. Characterization of DNA polymerase induced by bacteriophage T5 with DNA containing single strand breaks. J Biol Chem. 1976 Apr 10;251(7):2168–2174. [PubMed] [Google Scholar]
  8. Goulian M., Lucas Z. J., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. J Biol Chem. 1968 Feb 10;243(3):627–638. [PubMed] [Google Scholar]
  9. KILHAM L., MOLONEY J. B. ASSOCIATION OF RAT VIRUS AND MOLONEY LEUKEMIA VIRUS IN TISSUES OF INOCULATED RATS. J Natl Cancer Inst. 1964 Feb;32:523–531. [PubMed] [Google Scholar]
  10. KILHAM L., OLIVIER L. J. A latent virus of rats isolated in tissue culture. Virology. 1959 Apr;7(4):428–437. doi: 10.1016/0042-6822(59)90071-6. [DOI] [PubMed] [Google Scholar]
  11. Kasamo K., Shimomura T. The role of the epidermis in local lesion formation and the multiplication of tobacco mosaic virus and its relation to kinetin. Virology. 1977 Jan;76(1):12–18. doi: 10.1016/0042-6822(77)90276-8. [DOI] [PubMed] [Google Scholar]
  12. McMaster G. K., Beard P., Engers H. D., Hirt B. Characterization of an immunosuppressive parvovirus related to the minute virus of mice. J Virol. 1981 Apr;38(1):317–326. doi: 10.1128/jvi.38.1.317-326.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mitra S., Stallions D. R. The role of Escherichia coli dna A gene and its integrative suppression in M13 coliphage DNA synthesis. Eur J Biochem. 1976 Aug 1;67(1):37–45. doi: 10.1111/j.1432-1033.1976.tb10629.x. [DOI] [PubMed] [Google Scholar]
  14. Rhode S. L., 3rd Replication process of the parvovirus H-1. IX. Physical mapping studies of the H-1 genome. J Virol. 1977 May;22(2):446–458. doi: 10.1128/jvi.22.2.446-458.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rhode S. L., 3rd Replication process of the parvovirus H-1. X. Isolation of a mutant defective in replicative-form DNA replication. J Virol. 1978 Jan;25(1):215–223. doi: 10.1128/jvi.25.1.215-223.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salzman L. A., Fabisch P. Nucleotide sequence of the self-priming 3' terminus of the single-stranded DNA extracted from the parvovirus Kilham rat virus. J Virol. 1979 Jun;30(3):946–950. doi: 10.1128/jvi.30.3.946-950.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salzman L. A., Fabisch P., Parr R., Garon C., Wali T. In vitro synthesis of double-stranded DNA from the Kilham rat virus single-stranded DNA genome. J Virol. 1978 Sep;27(3):784–790. doi: 10.1128/jvi.27.3.784-790.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salzman L. A., White W. L., Kakefuda T. Linear, single-stranded deoxyribonucleic acid isolated from Kilham rat virus. J Virol. 1971 Jun;7(6):830–835. doi: 10.1128/jvi.7.6.830-835.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siegl G. The parvoviruses. Virol Monogr. 1976;15:1–109. [PubMed] [Google Scholar]
  20. Straus S. E., Sebring E. D., Rose J. A. Concatemers of alternating plus and minus strands are intermediates in adenovirus-associated virus DNA synthesis. Proc Natl Acad Sci U S A. 1976 Mar;73(3):742–746. doi: 10.1073/pnas.73.3.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tattersall P., Ward D. C. Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature. 1976 Sep 9;263(5573):106–109. doi: 10.1038/263106a0. [DOI] [PubMed] [Google Scholar]
  22. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES