Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1981 Nov;40(2):465–471. doi: 10.1128/jvi.40.2.465-471.1981

Excision repair and patch size in UV-irradiated bacteriophage T4.

D B Yarosh, B S Rosenstein, R B Setlow
PMCID: PMC256648  PMID: 6895652

Abstract

We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.

Full text

PDF
465

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed F. E., Setlow R. B. DNA repair in V-79 cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. Cancer Res. 1977 Sep;37(9):3414–3419. [PubMed] [Google Scholar]
  2. Ahmed F. E., Setlow R. B. DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. Cancer Res. 1979 Feb;39(2 Pt 1):471–479. [PubMed] [Google Scholar]
  3. Carrier W. L., Setlow R. B. Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties. J Bacteriol. 1970 Apr;102(1):178–186. doi: 10.1128/jb.102.1.178-186.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cook K. H., Friedberg E. C. Measurement of thymine dimers in DNA by thin-layer chromatography. II. The use of one-dimensional systems. Anal Biochem. 1976 Jun;73(2):411–418. doi: 10.1016/0003-2697(76)90188-3. [DOI] [PubMed] [Google Scholar]
  5. Cooper P. K., Hanawalt P. C. Heterogeneity of patch size in repair replicated DNA in Escherichia coli. J Mol Biol. 1972 Jun 14;67(1):1–10. doi: 10.1016/0022-2836(72)90381-6. [DOI] [PubMed] [Google Scholar]
  6. Cooper P. K., Hanawalt P. C. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1156–1160. doi: 10.1073/pnas.69.5.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedberg E. C. DNA repair of ultraviolet-irradiated bacteriophage T4. Photochem Photobiol. 1975 Apr;21(4):277–289. doi: 10.1111/j.1751-1097.1975.tb06670.x. [DOI] [PubMed] [Google Scholar]
  8. Friedberg E. C., King J. J. Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. J Bacteriol. 1971 May;106(2):500–507. doi: 10.1128/jb.106.2.500-507.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Friedberg E. C., King J. J. Endonucleolytic cleavage of UV-irradiated DNA controlled by the V+ gene in phage T4. Biochem Biophys Res Commun. 1969 Nov 6;37(4):646–651. doi: 10.1016/0006-291x(69)90859-6. [DOI] [PubMed] [Google Scholar]
  10. Grossman L., Braun A., Feldberg R., Mahler I. Enzymatic repair of DNA. Annu Rev Biochem. 1975;44:19–43. doi: 10.1146/annurev.bi.44.070175.000315. [DOI] [PubMed] [Google Scholar]
  11. Grossman L., Riazuddin S., Haseltine W. A., Lindan C. Nucleotide excision repair of damaged DNA. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):947–955. doi: 10.1101/sqb.1979.043.01.104. [DOI] [PubMed] [Google Scholar]
  12. HARM W. Mutants of phage T4 with increased sensitivity to ultraviolet. Virology. 1963 Jan;19:66–71. doi: 10.1016/0042-6822(63)90025-4. [DOI] [PubMed] [Google Scholar]
  13. Hartman P. E. Bacterial mutagenesis: review of new insights. Environ Mutagen. 1980;2(1):3–16. doi: 10.1002/em.2860020103. [DOI] [PubMed] [Google Scholar]
  14. Ley R. D., Setlow R. B. Repair replication in Escherichia coli as measured by the photolysis of bromodeoxyuridine. Biophys J. 1972 Apr;12(4):420–431. doi: 10.1016/S0006-3495(72)86094-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maynard-Smith S., Symonds N. Involvement of bacteriophage T4 genes in radiation repair. J Mol Biol. 1973 Feb 15;74(1):33–44. doi: 10.1016/0022-2836(73)90352-5. [DOI] [PubMed] [Google Scholar]
  16. Pawl G., Taylor R., Minton K., Friedberg E. C. Enzymes involved in thymine dimer excision in bacteriophage T4-infected Escherichia coli. J Mol Biol. 1976 Nov;108(1):99–109. doi: 10.1016/s0022-2836(76)80097-6. [DOI] [PubMed] [Google Scholar]
  17. Regan J. D., Setlow R. B., Ley R. D. Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine. Proc Natl Acad Sci U S A. 1971 Apr;68(4):708–712. doi: 10.1073/pnas.68.4.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Regan J. D., Setlow R. B. Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens. Cancer Res. 1974 Dec;34(12):3318–3325. [PubMed] [Google Scholar]
  19. Rothman R. H. Dimer excision and repair replication patch size in recL152 mutant of Escherichia coli K-12. J Bacteriol. 1978 Oct;136(1):444–448. doi: 10.1128/jb.136.1.444-448.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rothman R. H. Dimer excision in Escherichia coli in the presence of caffeine. J Bacteriol. 1980 Jul;143(1):520–524. doi: 10.1128/jb.143.1.520-524.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Seawell P. C., Simon T. J., Ganesan A. K. Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light. Biochemistry. 1980 Apr 15;19(8):1685–1691. doi: 10.1021/bi00549a026. [DOI] [PubMed] [Google Scholar]
  22. Seawell P. C., Smith C. A., Ganesan A. K. den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA. J Virol. 1980 Sep;35(3):790–796. doi: 10.1128/jvi.35.3.790-796.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Warner H. R., Demple B. F., Deutsch W. A., Kane C. M., Linn S. Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4602–4606. doi: 10.1073/pnas.77.8.4602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yasuda S., Sekiguchi M. Further purification and characterization of T4 endonuclease V. Biochim Biophys Acta. 1976 Aug 18;442(2):197–207. doi: 10.1016/0005-2787(76)90490-1. [DOI] [PubMed] [Google Scholar]
  25. Yasuda S., Sekiguchi M. T4 endonuclease involved in repair of DNA. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839–1845. doi: 10.1073/pnas.67.4.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES