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. Author manuscript; available in PMC: 2008 Oct 13.
Published in final edited form as: Biochemistry. 2008 Mar 15;47(14):4317–4328. doi: 10.1021/bi702409t

Figure 4.

Figure 4

Analysis of MTSET labeling of MscL by electrospray mass spectrometry. The G22C mutant of E. coli MscL was reconstituted into lipid vesicles containing DOPC and reacted with MTSET as described under Materials and Methods. (A) and (B) show electrospray mass spectra for MscL unreacted (A) and after reaction with MTSET (B). In (A) the peaks at molecular masses 16492 and 16361 correspond to MscL and MscL lacking the N-terminal Met residue, respectively. In (B) the peaks at molecular masses 16611 and 16480 correspond to labeled MscL and labeled MscL lacking the N-terminal Met residue, respectively, and the peaks at 16627 and 16496 correspond to single Met oxidations of MscL (MscL is Met-rich). No unlabeled MscL is observed in spectrum B.