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. Author manuscript; available in PMC: 2008 Oct 13.
Published in final edited form as: Biochemistry. 2007 May 1;46(20):5972–5981. doi: 10.1021/bi062053k

Figure 2.

Figure 2

Absorption and fluorescence spectra of ALAS and H282A variant. (A) UV-visible absorption spectra. The inset includes the region from 250–300 nm. Protein concentrations were adjusted to 13 μM in 20 mM Hepes, pH 7.5. (B) Fluorescence emission spectra of 5 μM ALAS and H282A in 20 mM Hepes, pH 7.5 containing 10% glycerol upon excitation at (B) 330 nm and (C) 420 nm. For (A)–(C), ALAS (--) and H282A (—).