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. 1981 Dec;40(3):920–926. doi: 10.1128/jvi.40.3.920-926.1981

5'-terminal deletions are a common feature of endogenous retrovirus loci located on chromosome 1 of White Leghorn chickens.

A Tereba
PMCID: PMC256703  PMID: 7321106

Abstract

My previous studies demonstrated that chromosome 1 has all five of the endogenous retrovirus loci associated with nonexpression of viral proteins (gs- chf-) in White Leghorn chickens. The current study was done to localize the two defective endogenous retrovirus loci, ev3 and ev6, to determine whether nonexpression of the viral loci on chromosome 1 is a transcriptional prerequisite or a result of an underlying structural defect. The structure of ev6 is every similar to that of two other gs- chf- -associated loci, ev4 and ev5; all three contain a 5'-terminal deletion that eliminates the viral transcriptional promoter. Unlike the gs- chf- -associated loci, ev6 apparently uses an adjacent cell promoter to produce high levels of viral glycoprotein. The ev6 locus was located on the long arm of chromosome 1 by in situ hybridization, thus indicating that transcription of an endogenous retrovirus locus on chromosome 1 can occur. However, the 5'-terminal deletion in several viral loci on chromosome 1, but not on other chromosomes, suggests that the deletion may result from the events that generate or maintain these loci in the White Leghorn population. The additional finding of ev3, which has an internal deletion, on a microchromosome suggests that the mechanism that produced deletions in many chromosome 1 loci is specific for the 5' terminus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astrin S. M. Endogenous viral genes of the White Leghorn chicken: common site of residence and sites associated with specific phenotypes of viral gene expression. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5941–5945. doi: 10.1073/pnas.75.12.5941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Astrin S. M., Robinson H. L., Crittenden L. B., Buss E. G., Wyban J., Hayward W. S. Ten genetic loci in the chicken that contain structural genes for endogenous avian leukosis viruses. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1105–1109. doi: 10.1101/sqb.1980.044.01.119. [DOI] [PubMed] [Google Scholar]
  3. Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frisby D. P., Weiss R. A., Roussel M., Stehelin D. The distribution of endogenous chicken retrovirus sequences in the DNA of galliform birds does not coincide with avian phylogenetic relationships. Cell. 1979 Jul;17(3):623–634. doi: 10.1016/0092-8674(79)90270-8. [DOI] [PubMed] [Google Scholar]
  5. Hayward W. S., Braverman S. B., Astrin S. M. Transcriptional products and DNA structure of endogenous avian proviruses. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1111–1121. doi: 10.1101/sqb.1980.044.01.120. [DOI] [PubMed] [Google Scholar]
  6. Hishinuma F., DeBona P. J., Astrin S., Skalka A. M. Nucleotide sequence of acceptor site and termini of integrated avian endogenous provirus ev1: integration creates a 6 bp repeat of host DNA. Cell. 1981 Jan;23(1):155–164. doi: 10.1016/0092-8674(81)90280-4. [DOI] [PubMed] [Google Scholar]
  7. Hughes S. H., Shank P. R., Spector D. H., Kung H. J., Bishop J. M., Varmus H. E., Vogt P. K., Breitman M. L. Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites. Cell. 1978 Dec;15(4):1397–1410. doi: 10.1016/0092-8674(78)90064-8. [DOI] [PubMed] [Google Scholar]
  8. Hughes S. H., Toyoshima K., Bishop J. M., Varmus H. E. Organization of the endogenous proviruses of chickens: implications for origin and expression. Virology. 1981 Jan 15;108(1):189–207. doi: 10.1016/0042-6822(81)90538-9. [DOI] [PubMed] [Google Scholar]
  9. Ju G., Skalka A. M. Nucleotide sequence analysis of the long terminal repeat (LTR) of avian retroviruses: structural similarities with transposable elements. Cell. 1980 Nov;22(2 Pt 2):379–386. doi: 10.1016/0092-8674(80)90348-7. [DOI] [PubMed] [Google Scholar]
  10. Lemons R. S., Nash W. G., O'Brien S. J., Benveniste R. E., Sherr C. J. A gene (Bevi) on human chromosome 6 is an integration site for baboon type C DNA provirus in human cells. Cell. 1978 Aug;14(4):995–1005. doi: 10.1016/0092-8674(78)90353-7. [DOI] [PubMed] [Google Scholar]
  11. Neiman P. E. Measurement of endogenous leukosis virus nucleotide sequences in the DNA of normal avian embryos by RNA-DNA hybridization. Virology. 1973 May;53(1):196–203. doi: 10.1016/0042-6822(73)90478-9. [DOI] [PubMed] [Google Scholar]
  12. Rosenthal P. N., Robinson H. L., Robinson W. S., Hanafusa T., Hanafusa H. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336–2340. doi: 10.1073/pnas.68.10.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sabran J. L., Hsu T. W., Yeater C., Kaji A., Mason W. S., Taylor J. M. Analysis of integrated avian RNA tumor virus DNA in transformed chicken, duck and quail fibroblasts. J Virol. 1979 Jan;29(1):170–178. doi: 10.1128/jvi.29.1.170-178.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shoemaker C., Goff S., Gilboa E., Paskind M., Mitra S. W., Baltimore D. Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3932–3936. doi: 10.1073/pnas.77.7.3932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Temin H. M. Origin of retroviruses from cellular moveable genetic elements. Cell. 1980 Oct;21(3):599–600. doi: 10.1016/0092-8674(80)90420-1. [DOI] [PubMed] [Google Scholar]
  16. Tereba A., Astrin S. M. Chromosomal localization of ev-1, a frequently occurring endogenous retrovirus locus in white Leghorn chickens, by in situ hybridization. J Virol. 1980 Sep;35(3):888–894. doi: 10.1128/jvi.35.3.888-894.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tereba A., Crittenden L. B., Astrin S. M. Chromosomal localization of three endogenous retrovirus loci associated with virus production in White Leghorn chickens. J Virol. 1981 Jul;39(1):282–289. doi: 10.1128/jvi.39.1.282-289.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tereba A., Lai M. M., Murti K. G. Chromosome 1 contains the endogenous RAV-0 retrovirus sequences in chicken cells. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6486–6490. doi: 10.1073/pnas.76.12.6486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tereba A., Skoog L., Vogt P. K. RNA tumor virus specific sequences in nuclear DNA of several avian species. Virology. 1975 Jun;65(2):524–534. doi: 10.1016/0042-6822(75)90057-4. [DOI] [PubMed] [Google Scholar]
  20. Varmus H. E., Bishop J. M., Vogt P. K. Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus. J Mol Biol. 1973 Mar 15;74(4):613–626. doi: 10.1016/0022-2836(73)90052-1. [DOI] [PubMed] [Google Scholar]
  21. Weinberg R. A. Integrated genomes of animal viruses. Annu Rev Biochem. 1980;49:197–226. doi: 10.1146/annurev.bi.49.070180.001213. [DOI] [PubMed] [Google Scholar]
  22. Weiss R. A., Biggs P. M. Leukosis and Marek's disease viruses of feral red jungle flow and domestic fowl in Malaya. J Natl Cancer Inst. 1972 Dec;49(6):1713–1725. doi: 10.1093/jnci/49.6.1713. [DOI] [PubMed] [Google Scholar]

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