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. Author manuscript; available in PMC: 2009 Aug 19.
Published in final edited form as: Toxicology. 2008 Jun 5;250(1):47–54. doi: 10.1016/j.tox.2008.05.018

Figure 1.

Figure 1

MTT cytotoxicity of antioxidants, KI and melatonin. A) 24 and 48 h toxicity tests of 10.0, 5.0, and 2.5 mM KI and melatonin in UROtsa cells (N=3). B & C) Trypan blue exclusion assay to determine cytotoxicity associated with co-treatment of arsenicals with ROS scavengers in UROtsa cells (N=3). B) Co-treatment of 1 μM As(III) and ROS scavengers does not cause a decrease in cell viability in UROtsa cells after 24 hour exposure when compared to As(III) treated alone group. C) Treatment with 50 nM MMA(III) with or without ROS scavengers has no significant effect on cytoxicity in UROtsa cells after 24 hour exposure when compared to MMA(III) treatment alone. ROS scavenger concentrations (vehicle) and abbreviations as follows: CAT-100 units/ml media of catalase; GSH-5 mM glutathione (H2O); NaN3- 1 mM sodium azide (H2O); KI-1 mM potassium iodide(H2O); melatonin-1 mM melatonin (EtOH); SOD- 100 units/ml media of superoxide dismutase. Cytotoxicity was also measured after 48 h with similar results (data not shown).