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. 1982 Jan;41(1):97–103. doi: 10.1128/jvi.41.1.97-103.1982

Shope Fibroma Virus I. Biological and Molecular Properties of a Cytocidal and a Noncytocidal Strain

Beatriz G T Pogo 1, Paul Freimuth 1, Adriane Stein 1
PMCID: PMC256729  PMID: 6283131

Abstract

The biological and molecular properties of two strains of Shope fibroma virus (SFV) were compared. SFV-I was highly cytocidal to most of the cell lines tested and produced pocks in the chorioallantoic membrane of chick embryos. By contrast, SFV-W did not produce cytopathic effects in any of the cell lines or in the chorioallantoic membrane, but it induced characteristic foci 3 to 4 days after infection. Both strains produced tumors when inoculated into the skin of susceptible rabbits. Maximal infectivity in BSC-1 cells was reached by both strains between 24 to 48 h after inoculation. Viral DNA synthesis also took place at the same time, although cells infected with SFV-I incorporated three times more [3H]thymidine than cells infected with SFV-W. Sedimentation analysis and hydroxylapatite chromatography of the two viral DNAs indicated that their molecular weights were similar and that both were naturally cross-linked. Digestion with three restriction endonucleases, however, revealed that they had different restriction sites. When SFV-I and vaccinia DNA were compared, the restriction patterns were more alike. Analysis of the virion structural proteins by gel electrophoresis indicated that SFV-I, SFV-W, and vaccinia virus had many polypeptides in common, although there were distinctive differences among the three viruses. Finally, the results of plaque neutralization tests with different antisera showed that SFV-I and SFV-W shared common antigens and that vaccinia antiserum inhibited SFV-I but not SFV-W. We conclude that the SFV-I genome contains information for both cytolysis and tumorigenesis. This unusual virus may be a recombinant between an orthopoxvirus and a leporipoxvirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DALES S. The uptake and development of vaccinia virus in strain L cells followed with labeled viral deoxyribonucleic acid. J Cell Biol. 1963 Jul;18:51–72. doi: 10.1083/jcb.18.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ewton D., Hodes M. E. Nucleic acid synthesis in HeLa cells infected with Shope fibroma virus. Virology. 1967 Sep;33(1):77–83. doi: 10.1016/0042-6822(67)90095-5. [DOI] [PubMed] [Google Scholar]
  3. HINZE H. C., WALKER D. L. RESPONSE OF CULTURED RABBIT CELLS TO INFECTION WITH THE SHOPE FIBROMA VIRUS. I. PROLIFERATION AND MORPHOLOGICAL ALTERATION OF THE INFECTED CELLS. J Bacteriol. 1964 Oct;88:1185–1194. doi: 10.1128/jb.88.4.1185-1194.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hinze H. C., Walker D. L. Comparison of cytocidal and noncytocidal strains of Shope rabbit fibroma virus. J Virol. 1971 May;7(5):577–581. doi: 10.1128/jvi.7.5.577-581.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacquemont B., Gazzolo L. Existence d'une synth'ese de DNA cellulaire liée à l'infection virale dans les cellules infectées par le virus du fibrome de Shope. C R Acad Sci Hebd Seances Acad Sci D. 1971 Jul 12;273(2):253–256. [PubMed] [Google Scholar]
  6. OVERMAN J. R., TAMM I. Multiplication of vaccinia virus in the chorioallantoic membrane in vitro. Virology. 1957 Feb;3(1):173–184. doi: 10.1016/0042-6822(57)90031-4. [DOI] [PubMed] [Google Scholar]
  7. Padgett B. L., Walker D. L. Effect of persistent fibroma virus infection on susceptibility of cells to other viruses. J Virol. 1970 Feb;5(2):199–204. doi: 10.1128/jvi.5.2.199-204.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pogo B. G. Changes in parental vaccinia virus DNA after viral penetration into cells. Virology. 1980 Mar;101(2):520–524. doi: 10.1016/0042-6822(80)90466-3. [DOI] [PubMed] [Google Scholar]
  9. Pogo B. G., Dales S. Biogenesis of poxviruses: further evidence for inhibition of host and virus DNA synthesis by a component of the invading inoculum particle. Virology. 1974 Apr;58(2):377–386. doi: 10.1016/0042-6822(74)90073-7. [DOI] [PubMed] [Google Scholar]
  10. Pogo B. G. Elimination of naturally occurring crosslinks in vaccinia virus DNA after viral penetration into cells. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1739–1742. doi: 10.1073/pnas.74.4.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pogo B. G., O'Shea M. T. The mode of replication of vaccinia virus DNA. Virology. 1978 Jan;84(1):1–8. doi: 10.1016/0042-6822(78)90213-1. [DOI] [PubMed] [Google Scholar]
  12. Pogo B. G., O'Shea M., Freimuth P. Initiation and termination of vaccinia virus DNA replication. Virology. 1981 Jan 15;108(1):241–248. doi: 10.1016/0042-6822(81)90543-2. [DOI] [PubMed] [Google Scholar]
  13. Pogo B. G., Stein A., Freimuth P. Shope fibroma virus. II. Role of the virion-associated nucleases. J Virol. 1982 Jan;41(1):104–109. doi: 10.1128/jvi.41.1.104-109.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tompkins W. A., Walker D. L., Hinze H. C. Cellular deoxyribonucleic acid synthesis and loss of contact inhibition in irradiated and contact-inhibited cell cultures infected with fibroma virus. J Virol. 1969 Nov;4(5):603–609. doi: 10.1128/jvi.4.5.603-609.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. des Gouttes Olgiati D., Pogo B. G., Dales S. Biogenesis of vaccinia: specific inhibition of rapidly labeled host DNA in vaccinia inoculated cells. Virology. 1976 May;71(1):325–335. doi: 10.1016/0042-6822(76)90116-1. [DOI] [PubMed] [Google Scholar]

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