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. 1982 Jan;41(1):145–152. doi: 10.1128/jvi.41.1.145-152.1982

Characterization of N-type and dually permissive cells segregated from mouse fibroblasts whose Fv-1 phenotype could be modified by another independently segregating gene(s).

H Yoshikura, S Tejima, T Kuchino, K Segawa, T Odaka
PMCID: PMC256735  PMID: 6283109

Abstract

Though the inbred DDD mouse strain is essentially of the N type, the primary culture of this strain was about 100-fold more sensitive to B-tropic WN1802B virus than were the typical N-type strains (C3H/He, C57L, etc.). After cloning, DDD mouse cells segregated two types of cells, typical N-type cells and cells lacking in Fv-1 restriction. As both types of cells so far tested retained glucose-6-phosphatase-1 coded by a locus closely linked to Fv-1 and genetic cross experiments indicated the presence of a gene(s) modifying the Fv-1 phenotype, variation in Fv-1 restriction could presumably be brought about by genetic changes in a gene(s) other than Fv-1 itself. N-type and dually permissive cell clones were similarly established from the inbred G mouse. Compositions of polypeptides labeled with [35S]methionine in the N-type and dually permissive cells of DDD and G mouse origins were compared by two-dimensional gel electrophoresis. The polypeptide maps of these cells were similar except for a few spots. Among these dissimilar spots, a spot of about 20,000 daltons with a pI of about 5.5 was always present in N-type cells, whereas it was absent in dually permissive cells. In DDD mouse-derived clones, a proportional relation was observed between the intensity of the spot and the restriction to the B-tropic virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelrad A. Genetic and cellular basis of susceptibility or resistance to Friend leukemia virus infection in mice. Proc Can Cancer Conf. 1969;8:313–343. [PubMed] [Google Scholar]
  2. Declève A., Niwa O., Kojola J., Kaplan H. S. New gene locus modifying susceptibility to certain B-tropic murine leukemia viruses. Proc Natl Acad Sci U S A. 1976 Feb;73(2):585–590. doi: 10.1073/pnas.73.2.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  4. Huang A. S., Besmer P., Chu L., Baltimore D. Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses. J Virol. 1973 Sep;12(3):659–662. doi: 10.1128/jvi.12.3.659-662.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jolicoeur P., Baltimore D. Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2236–2240. doi: 10.1073/pnas.73.7.2236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jolicoeur P., Rassart E. Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus. J Virol. 1980 Jan;33(1):183–195. doi: 10.1128/jvi.33.1.183-195.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krontiris T. G., Soeiro R., Fields B. N. Host restriction of Friend leukemia virus. Role of the viral outer coat. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2549–2553. doi: 10.1073/pnas.70.9.2549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nichols E. A., Ruddle F. H. A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels. J Histochem Cytochem. 1973 Dec;21(12):1066–1081. doi: 10.1177/21.12.1066. [DOI] [PubMed] [Google Scholar]
  9. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  10. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. IV. Dose-response relationships in Fv-1-sensitive and resistant cell cultures. Virology. 1975 Jun;65(2):333–342. doi: 10.1016/0042-6822(75)90039-2. [DOI] [PubMed] [Google Scholar]
  11. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  12. Rowe W. P., Sato H. Genetic mapping of the Fv-1 lcous of the mouse. Science. 1973 May 11;180(4086):640–641. doi: 10.1126/science.180.4086.640. [DOI] [PubMed] [Google Scholar]
  13. Suzuki S., Matsubara S. Isolation of Friend leukemia virus resistant line from non-inbred mouse colony. Jpn J Exp Med. 1975 Dec;45(6):467–471. [PubMed] [Google Scholar]
  14. Switzer R. C., 3rd, Merril C. R., Shifrin S. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem. 1979 Sep 15;98(1):231–237. doi: 10.1016/0003-2697(79)90732-2. [DOI] [PubMed] [Google Scholar]
  15. Tennant R. W., Schluter B., Myer F. E., Otten J. A., Yang W. K., Brown A. Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses. J Virol. 1976 Dec;20(3):589–596. doi: 10.1128/jvi.20.3.589-596.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang W. K., Kiggans J. O., Yang D. M., Ou C. Y., Tennant R. W., Brown A., Bassin R. H. Synthesis and circularization of N- and B-tropic retroviral DNA Fv-1 permissive and restrictive mouse cells. Proc Natl Acad Sci U S A. 1980 May;77(5):2994–2998. doi: 10.1073/pnas.77.5.2994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yoshikura H. Host range conversion of the murine sarcoma-leukaemia complex. J Gen Virol. 1973 Jun;19(3):321–327. doi: 10.1099/0022-1317-19-3-321. [DOI] [PubMed] [Google Scholar]
  18. Yoshikura H., Naito Y., Moriwaki K. Unstable resistance of G mouse fibroblasts to ecotropic murine leukemia virus infection. J Virol. 1979 Mar;29(3):1078–1086. doi: 10.1128/jvi.29.3.1078-1086.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoshikura H. Ultraviolet inactivation of murine leukemia virus and its assay in permissive and non-permissive cells. Int J Cancer. 1973 May;11(3):739–746. doi: 10.1002/ijc.2910110325. [DOI] [PubMed] [Google Scholar]
  20. Yoshikura H., Yoshida M. Intracellular restriction of ecotropic murine leukemia virus in rat NRK cells and its abolishment by adaptation. J Virol. 1978 Sep;27(3):612–618. doi: 10.1128/jvi.27.3.612-618.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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