Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1982 Jan;41(1):228–236. doi: 10.1128/jvi.41.1.228-236.1982

Cloning and characterization of an envelope-specific probe from xenotropic murine leukemia proviral DNA.

C E Buckler, M D Hoggan, H W Chan, J F Sears, A S Khan, J L Moore, J W Hartley, W P Rowe, M A Martin
PMCID: PMC256743  PMID: 6283115

Abstract

An 8.9-kilobase EcoRI restriction fragment was cloned from mink cells chronically infected with NFS-Th-1 xenotropic murine leukemia virus by using a lambda phage host vector system. After its transfer into pBR322, the EcoRI DNA insert was characterized and found to contain 6.7 kilobases of proviral DNA sequences and 2.2 kilobases of mink cellular DNA flanking the 5' end of the viral genome. A 500-base pair fragment which was located at the 3' terminus of the cloned DNA insert and which mapped to the env region of xenotropic proviral DNA was subcloned into pBR322. This xenotropic envelope proviral DNA segment did not hybridize to ecotropic murine leukemia proviruses but did anneal to representative alpha and beta xenotropic and seven different mink cell focus-inducing proviral DNAs. The cloned xenotropic envelope-specific probe was also used in blot hybridization experiments to analyze the arrangement of related sequences in preparations of different mouse liver DNAs.

Full text

PDF
228

Images in this article

230Image
on p.230
232Image
on p.232
233Image
on p.233
234Image
on p.234

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Benveniste R. E., Lieber M. M., Todaro G. J. A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line. Proc Natl Acad Sci U S A. 1974 Mar;71(3):602–606. doi: 10.1073/pnas.71.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bosselman R. A., Van Griensven L. J., Vogt M., Verma I. M. Genome organization of retroviruses. VI. Heteroduplex analysis of ecotropic and xenotropic sequences of moloney mink cell focus-inducing viral RNA obtained from either a cloned isolate or a thymoma cell line. J Virol. 1979 Dec;32(3):968–978. doi: 10.1128/jvi.32.3.968-978.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Callahan R., Lieber M. M., Todaro G. J. Nucleic acid homology of murine xenotropic type C viruses. J Virol. 1975 Jun;15(6):1378–1384. doi: 10.1128/jvi.15.6.1378-1384.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chan H. W., Bryan T., Moore J. L., Staal S. P., Rowe W. P., Martin M. A. Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5779–5783. doi: 10.1073/pnas.77.10.5779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. Qualitative and quantitative studies of AKR-type murine leukemia virus sequences in mouse DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1085–1101. doi: 10.1101/sqb.1974.039.01.124. [DOI] [PubMed] [Google Scholar]
  7. Chien Y. H., Verma I. M., Shih T. Y., Scolnick E. M., Davidson N. Heteroduplex analysis of the sequence relations between the RNAs of mink cell focus-inducing and murine leukemia viruses. J Virol. 1978 Oct;28(1):352–360. doi: 10.1128/jvi.28.1.352-360.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cloyd M. W., Hartley J. W., Rowe W. P. Cell-surface antigens associated with recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1979 Mar 1;149(3):702–712. doi: 10.1084/jem.149.3.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  10. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Chused T. M., Morse H. C., Hartley J. W., Rowe W. P. Differential expression of two distinct xenotropic viruses in NZB mice. Clin Immunol Immunopathol. 1980 Mar;15(3):493–501. doi: 10.1016/0090-1229(80)90061-6. [DOI] [PubMed] [Google Scholar]
  11. Elder J. H., Jensen F. C., Bryant M. L., Lerner R. A. Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family. Nature. 1977 May 5;267(5606):23–28. doi: 10.1038/267023a0. [DOI] [PubMed] [Google Scholar]
  12. Gerwin B. I., Smith S. G., Peebles P. T. Two active forms of RD-114 virus DNA polymerase in infected cells. Cell. 1975 Sep;6(1):45–52. doi: 10.1016/0092-8674(75)90072-0. [DOI] [PubMed] [Google Scholar]
  13. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hager G. L., Chang E. H., Chan H. W., Garon C. F., Israel M. A., Martin M. A., Scolnick E. M., Lowy D. R. Molecular cloning of the Harvey sarcoma virus closed circular DNA intermediates: initial structural and biological characterization. J Virol. 1979 Sep;31(3):795–809. doi: 10.1128/jvi.31.3.795-809.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  18. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  19. Ishimoto A., Hartley J. W., Rowe W. P. Phenotypic mixing between murine leukemia viruses: characteristics of ecotropic virus infection of heterologous cells. Virology. 1978 Dec;91(2):464–471. doi: 10.1016/0042-6822(78)90392-6. [DOI] [PubMed] [Google Scholar]
  20. Israel M. A., Vanderryn D. F., Meltzer M. L., Martin M. A. Characterization of polyoma viral DNA sequences in polyoma-induced hamster tumor cell lines. J Biol Chem. 1980 Apr 25;255(8):3798–3805. [PubMed] [Google Scholar]
  21. Karshin W. L., Arcement L. J., Naso R. B., Arlinghaus R. B. Common precursor for Rauscher leukemia virus gp69/71, p15(E), and p12(E). J Virol. 1977 Sep;23(3):787–798. doi: 10.1128/jvi.23.3.787-798.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kozak C. A., Rowe W. P. Genetic mapping of xenotropic murine leukemia virus-inducing loci in five mouse strains. J Exp Med. 1980 Jul 1;152(1):219–228. doi: 10.1084/jem.152.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lowy D. R., Rands E., Chattopadhyay S. K., Garon C. F., Hager G. L. Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):614–618. doi: 10.1073/pnas.77.1.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Marcus S. L. Resolution and characterization of intracytoplasmic forms of reverse transcriptase from Rauscher leukemia virus-producing cells. J Virol. 1978 Apr;26(1):1–10. doi: 10.1128/jvi.26.1.1-10.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Martin M. A., Bryan T., McCutchan T. F., Chan H. W. Detection and cloning of murine leukemia virus-related sequences from African green monkey liver DNA. J Virol. 1981 Sep;39(3):835–844. doi: 10.1128/jvi.39.3.835-844.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  28. Morse H. C., 3rd, Chused T. M., Boehm-Truitt M., Mathieson B. J., Sharrow S. O., Hartley J. W. XenCSA: cell surface antigens related to the major glycoproteins (gp70) of xenotropic murine leukemia viruses. J Immunol. 1979 Feb;122(2):443–454. [PubMed] [Google Scholar]
  29. Rowe W. P. Leukemia virus genomes in the chromosomal DNA of the mouse. Harvey Lect. 1978;71:173–192. [PubMed] [Google Scholar]
  30. Schultz A. M., Rabin E. H., Oroszlan S. Post-translational modification of Rauscher leukemia virus precursor polyproteins encoded by the gag gene. J Virol. 1979 Apr;30(1):255–266. doi: 10.1128/jvi.30.1.255-266.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  32. Steffen D., Bird S., Rowe W. P., Weinberg R. A. Identification of DNA fragments carrying ecotropic proviruses of AKR mice. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4554–4558. doi: 10.1073/pnas.76.9.4554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Steffen D., Weinberg R. A. The integrated genome of murine leukemia virus. Cell. 1978 Nov;15(3):1003–1010. doi: 10.1016/0092-8674(78)90284-2. [DOI] [PubMed] [Google Scholar]
  34. Stephenson J. R., Aaronson S. A., Arnstein P., Huebner R. J., Tronick S. R. Demonstration of two immunologically distinct xenotropic type C RNA viruses of mouse cells. Virology. 1974 Sep;61(1):56–63. doi: 10.1016/0042-6822(74)90241-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES