PLANT BIOLOGY. For the article “Arabidopsis TAO1 is a TIR-NB-LRR protein that contributes to disease resistance induced by the Pseudomonas syringae effector AvrB,” by Timothy K. Eitas, Zachary L. Nimchuk, and Jeffery L. Dangl, which appeared in issue 17, April 29, 2008, of Proc Natl Acad Sci USA (105:6475–6480; first published April 18, 2008; 10.1073/pnas.0802157105), the authors note that an incorrect protein blot was inadvertently used in Fig. 1B. This error does not affect the conclusions of the article. The corrected figure and legend appear below.
Fig. 1.
TAO1 is a TIR-NB-LRR R protein required for AvrB-induced chlorosis in rpm1 host plants. (A) Mt-0 leaves were inoculated with Agrobacterium containing T-DNA with a DEX:avrB-HA transgene (4). Leaves were treated with DEX 48 h after inoculation. The picture was taken 72 h after inoculation. (B) Plants of TAO1 genotype shown above the blots, and carrying the transgenes shown below the blots, were sprayed with either DEX or carrier (see Methods), and tissue samples were harvested at 1, 2, and 3 days after treatment. Protein blots were probed with an anti-HA monoclonal antibody. (C) Deduced structure of TAO1 alleles recovered in Mt-0 (red and black), Col-0 Salk T-DNA insertion lines (blue), and as polymorphisms in Cvi-0 (purple). For all missense mutations, the wild-type Mt-0 residue is listed first. TIR, amino acids 42–172; NB, amino acids 197–492; LRR, amino acids 610-1122. All TAO1 alleles in Mt-0 were generated by EMS mutagenesis except tao1–8, which is a fast neutron deletion of 1 bp in codon S667. Red tao1 alleles represent alleles that were out-crossed away from the DEX:avrB-HA transgene and used for further analyses. The tao1–10 (Salk_124245) insertion begins at amino acid 168. The tao1–11 (Salk_011670) insertion begins at amino acid 597. The only amino acid difference between Mt-0 and Col-0 is V489M. Genomic TAO1 sequences for Mt-0 (EU031442) and Cvi-0 (EU031443) have been deposited in GenBank.