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. 1982 Feb;41(2):557–565. doi: 10.1128/jvi.41.2.557-565.1982

Further characterization of mRNA's of mouse hepatitis virus: presence of common 5'-end nucleotides.

M M Lai, C D Patton, S A Stohlman
PMCID: PMC256784  PMID: 6281467

Abstract

The mouse hepatitis virus strain A59 codes for seven RNA species in the infected cells. These virus-specific RNAs were found to be polysome associated and therefore likely to represent mRNA's. All of them have common 3'-end sequences (Lai et al., J. Virol. 39:823-834, 1981). Their structure was further studied with respect to their 5'-end sequences. It was found that all of these mRNA's contained cap structures at their 5' ends. Furthermore, the cap-containing oligonucleotides which represent the sequences immediately adjacent to the 5' ends were found to be the same for most, if not all, of the seven virus-specific mRNA's. These sequences are also identical to the 5'-end sequences of the virion RNA genome. The 5'-end sequences were tentatively determined to be 5'-cap-N-UAAG. The presence of the common nucleotides in all of the virus-specific RNAs in mouse hepatitis virus strain A59 suggests several possible mechanisms of synthesis for these RNAs. The significance of these findings is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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