Table 4.
Media optimization design
| Exp. no. | Factors | Response variables | Comment | |||||
|---|---|---|---|---|---|---|---|---|
| HGF (ng/ml) | OSM (ng/ml) | FGF4 (ng/ml) | Urea (mg/l) | Lac (mg/l) | LDH (U/l) | Alb (μg/ml) | ||
| 1 | 0 | 0 | 0 | 8.0 | 121 | 34.0 | 14.1 | |
| 2 | 40 | 0 | 0 | 11.0 | 123 | 44.0 | 12.0 | |
| 3 | 0 | 50 | 0 | 14.0 | 202 | 66.0 | 18.4 | |
| 4 | 40 | 50 | 0 | 14.0 | 135 | 86.0 | 7.13 | |
| 5 | 0 | 0 | 40 | 7.0 | 76.6 | 28.0 | 17.8 | |
| 6 | 40 | 0 | 40 | 8.0 | 134 | 21.0 | 9.8 | |
| 7 | 0 | 50 | 40 | 11.0 | 177 | 44.0 | 9.2 | |
| 8 | 40 | 50 | 40 | 14.0 | 130 | 127 | 2.6 | |
| 9 | 0 | 25 | 20 | 10.0 | 176 | 42.0 | 7.0 | |
| 10 | 40 | 25 | 20 | 13.0 | 140 | 95.0 | 5.3 | |
| 11 | 20 | 0 | 20 | 9.0 | 139 | 28.0 | 14.6 | |
| 12 | 20 | 50 | 20 | 9.0 | 185 | 43.0 | 5.8 | |
| 13 | 20 | 25 | 0 | 12.0 | 180 | 60.0 | 4.9 | |
| 14 | 20 | 25 | 40 | 9.0 | 186 | 47.0 | 4.9 | |
| 15 | 20 | 25 | 20 | 14.0 | 132 | 74.0 | 6.9 | Center point |
| 16 | 20 | 25 | 20 | 10.0 | 191 | 51.0 | 4.7 | Center point |
| 17 | 20 | 25 | 20 | 11.0 | 175 | 50.0 | 6.0 | Center point |
| 18 | 0 | 0 | 0 | 8.0 | 123 | 32.0 | 18.3 | Blocking exp |