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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Methods. 2008 Jun 27;45(3):185–191. doi: 10.1016/j.ymeth.2008.06.003

Figure 5. Strategy for BiFC analysis of temporal and spatial interactions in C. elegans using a smg inducible system.

Figure 5

Shown is a flow chart for BiFC analysis of temporal and spatial interactions using a smg inducible expression system. Note that the 3'UTR is from plasmid L3809 from the 1997 Fire Kit and that smg-1(cc546) I is from CGC. The promoter regions, gene of interests, and the sequences encoding VN173 and VC155 along with the linker sequences can be released or amplified from the constructs in Figure 1 and cloned into L3809.