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. 1992 Apr;60(4):1699–1702. doi: 10.1128/iai.60.4.1699-1702.1992

Effect of Brucella abortus lipopolysaccharide on oxidative metabolism and lysozyme release by human neutrophils.

O Rasool 1, E Freer 1, E Moreno 1, C Jarstrand 1
PMCID: PMC257051  PMID: 1548094

Abstract

Both Brucella abortus lipopolysaccharide (LPS) and lipid A were low activators of nitroblue tetrazolium reduction and lysozyme release in human neutrophils. The stimulation was dose dependent and was higher in the presence of autologous plasma than in its absence. The comparison between Brucella LPS and lipid A versus Salmonella LPS revealed that at least 100 times more LPS and 1,000 times more lipid A of the former genus were required to induce significant nitroblue tetrazolium reduction and a corresponding lysozyme release in neutrophils. Low Brucella LPS-mediated superoxide and lysozyme production might contribute to the survival of these facultative intracellular bacteria in phagocytic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman D. T., Kurtz R. S. Relationship of biological activities to structures of Brucella abortus endotoxin and LPS. Ann Inst Pasteur Microbiol. 1987 Jan-Feb;138(1):98–101. doi: 10.1016/0769-2609(87)90084-6. [DOI] [PubMed] [Google Scholar]
  2. Böyum A. Separation of leukocytes from blood and bone marrow. Introduction. Scand J Clin Lab Invest Suppl. 1968;97:7–7. [PubMed] [Google Scholar]
  3. Canning P. C., Roth J. A., Deyoe B. L. Release of 5'-guanosine monophosphate and adenine by Brucella abortus and their role in the intracellular survival of the bacteria. J Infect Dis. 1986 Sep;154(3):464–470. doi: 10.1093/infdis/154.3.464. [DOI] [PubMed] [Google Scholar]
  4. Caroff M., Bundle D. R., Perry M. B., Cherwonogrodzky J. W., Duncan J. R. Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3. Infect Immun. 1984 Nov;46(2):384–388. doi: 10.1128/iai.46.2.384-388.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cline M. J., Melmon K. L., Davis W. C., Williams H. E. Mechanism of endotoxin interaction with human leucocytes. Br J Haematol. 1968 Dec;15(6):539–547. doi: 10.1111/j.1365-2141.1968.tb01576.x. [DOI] [PubMed] [Google Scholar]
  6. Davies M., Stewart-Tull D. E. The affinity of bacterial polysaccharide-containing fractions for mammalian cell membranes and its relationship to immunopotentiating activity. Biochim Biophys Acta. 1981 Apr 22;643(1):17–29. doi: 10.1016/0005-2736(81)90215-7. [DOI] [PubMed] [Google Scholar]
  7. Detilleux P. G., Deyoe B. L., Cheville N. F. Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro. Infect Immun. 1990 Jul;58(7):2320–2328. doi: 10.1128/iai.58.7.2320-2328.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gallego M. C., Cuello F., Garrido A. In vitro determination of phagocytosis and intracellular killing of Brucella melitensis by goat's polymorphonuclear phagocytes. Comp Immunol Microbiol Infect Dis. 1989;12(1-2):9–15. doi: 10.1016/0147-9571(89)90004-0. [DOI] [PubMed] [Google Scholar]
  9. Hall W. H., Manion R. E. In vitro susceptibility of Brucella to various antibiotics. Appl Microbiol. 1970 Oct;20(4):600–604. doi: 10.1128/am.20.4.600-604.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jarstrand C. The NBT (Nitroblue Tetrazolium) activity of neutrophil granulocytes in patients with influenza A infection. Scand J Infect Dis. 1977;9(1):5–7. doi: 10.3109/inf.1977.9.issue-1.02. [DOI] [PubMed] [Google Scholar]
  11. KARLSBAD G., KESSEL R. W., DE PETRIS S., MONACO L. ELECTRON MICROSCOPE OBSERVATIONS OF BRUCELLA ABORTUS GROWN WITHIN MONOCYTES IN VITRO. J Gen Microbiol. 1964 Jun;35:383–390. doi: 10.1099/00221287-35-3-383. [DOI] [PubMed] [Google Scholar]
  12. Keleti G., Feingold D. S., Youngner J. S. Interferon induction in mice by lipopolysaccharide from Brucella abortus. Infect Immun. 1974 Jul;10(1):282–283. doi: 10.1128/iai.10.1.282-283.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kreutzer D. L., Dreyfus L. A., Robertson D. C. Interaction of polymorphonuclear leukocytes with smooth and rough strains of Brucella abortus. Infect Immun. 1979 Mar;23(3):737–742. doi: 10.1128/iai.23.3.737-742.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee C. M., Mayer E. P., Molnar J., Teodorescu M. The mechanism of natural binding of bacteria to human lymphocyte subpopulations. J Clin Lab Immunol. 1983 Jun;11(2):87–94. [PubMed] [Google Scholar]
  15. Mayer H., Krauss J. H., Yokota A., Weckesser J. Natural variants of lipid A. Adv Exp Med Biol. 1990;256:45–70. doi: 10.1007/978-1-4757-5140-6_3. [DOI] [PubMed] [Google Scholar]
  16. Merzbach D., Obedeanu N. Standardisation of the nitroblue-tetrazolium test. J Med Microbiol. 1975 May;8(2):375–384. doi: 10.1099/00222615-8-2-375. [DOI] [PubMed] [Google Scholar]
  17. Moreno E., Berman D. T., Boettcher L. A. Biological activities of Brucella abortus lipopolysaccharides. Infect Immun. 1981 Jan;31(1):362–370. doi: 10.1128/iai.31.1.362-370.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moreno E., Berman D. T. Brucella abortus lipopolysaccharide is mitogenic for spleen cells of endotoxin-resistant C3H/HeJ mice. J Immunol. 1979 Dec;123(6):2915–2919. [PubMed] [Google Scholar]
  19. Moreno E., Borowiak D., Mayer H. Brucella lipopolysaccharides and polysaccharides. Ann Inst Pasteur Microbiol. 1987 Jan-Feb;138(1):102–105. doi: 10.1016/0769-2609(87)90085-8. [DOI] [PubMed] [Google Scholar]
  20. Moreno E., Mayer H., Moriyon I. Characterization of a native polysaccharide hapten from Brucella melitensis. Infect Immun. 1987 Nov;55(11):2850–2853. doi: 10.1128/iai.55.11.2850-2853.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moreno E., Pitt M. W., Jones L. M., Schurig G. G., Berman D. T. Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus. J Bacteriol. 1979 May;138(2):361–369. doi: 10.1128/jb.138.2.361-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Moreno E., Speth S. L., Jones L. M., Berman D. T. Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides. Infect Immun. 1981 Jan;31(1):214–222. doi: 10.1128/iai.31.1.214-222.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moreno E., Stackebrandt E., Dorsch M., Wolters J., Busch M., Mayer H. Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria. J Bacteriol. 1990 Jul;172(7):3569–3576. doi: 10.1128/jb.172.7.3569-3576.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moriyon I., Berman D. T. Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope. J Bacteriol. 1982 Nov;152(2):822–828. doi: 10.1128/jb.152.2.822-828.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Raetz C. R. Biochemistry of endotoxins. Annu Rev Biochem. 1990;59:129–170. doi: 10.1146/annurev.bi.59.070190.001021. [DOI] [PubMed] [Google Scholar]
  26. Riley L. K., Robertson D. C. Ingestion and intracellular survival of Brucella abortus in human and bovine polymorphonuclear leukocytes. Infect Immun. 1984 Oct;46(1):224–230. doi: 10.1128/iai.46.1.224-230.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Saha A. K., Mukhopadhyay N. K., Dowling J. N., Ficht T. A., Adams L. G., Glew R. H. Characterization of a phosphomonoesterase from Brucella abortus. Infect Immun. 1990 May;58(5):1153–1158. doi: 10.1128/iai.58.5.1153-1158.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schramek S., Brezina R., Kazár J. Some biological properties of an endotoxic lipopolysaccharide from the typhus group rickettsiae. Acta Virol. 1977 Sep;21(5):439–441. [PubMed] [Google Scholar]
  29. Smith L. D., Ficht T. A. Pathogenesis of Brucella. Crit Rev Microbiol. 1990;17(3):209–230. doi: 10.3109/10408419009105726. [DOI] [PubMed] [Google Scholar]
  30. Springer G. F., Adye J. C. Endotoxin-binding substances from human leukocytes and platelets. Infect Immun. 1975 Nov;12(5):978–986. doi: 10.1128/iai.12.5.978-986.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Young E. J., Borchert M., Kretzer F. L., Musher D. M. Phagocytosis and killing of Brucella by human polymorphonuclear leukocytes. J Infect Dis. 1985 Apr;151(4):682–690. doi: 10.1093/infdis/151.4.682. [DOI] [PubMed] [Google Scholar]

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