Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Apr;60(4):1711–1713. doi: 10.1128/iai.60.4.1711-1713.1992

Modulation of a surface antigen of Entamoeba histolytica in response to bacteria.

A Bhattacharya 1, R Ghildyal 1, J Prasad 1, S Bhattacharya 1, L S Diamond 1
PMCID: PMC257054  PMID: 1548096

Abstract

Changes in the cell surface of Entamoeba histolytica, a human intestinal parasite and the causative agent of amebic dysentery, were examined with a monoclonal antibody, 2D7.10, which selectively recognizes carbohydrate epitopes in some axenic amebic strains. While high-level expression of this epitope was observed in axenic amebae, it was either absent or present only in small amounts in xenic amebae. Furthermore, reassociation of the axenic amebae with intestinal flora resulted in loss of the 2D7.10 epitope. Our data suggest that surface antigens of E. histolytica can be modulated in response to bacteria and may provide an explanation for the observed influence of bacteria on amebic virulence.

Full text

PDF
1711

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aust-Kettis A., Thorstensson R., Sundqvist K. G. Dynamics of the interaction between Entamoeba histolytica and components of the immune response. III. Fate of antibodies after binding to the cell surface. Scand J Immunol. 1981;13(5):473–481. doi: 10.1111/j.1365-3083.1981.tb00159.x. [DOI] [PubMed] [Google Scholar]
  2. Bhattacharya A., Bhattacharya S., Sharma M. P., Diamond L. S. Metabolic labeling of Entamoeba histolytica antigens: characterization of a 28-kDa major intracellular antigen. Exp Parasitol. 1990 Apr;70(3):255–263. doi: 10.1016/0014-4894(90)90107-n. [DOI] [PubMed] [Google Scholar]
  3. Bhattacharya A., Ghildyal R., Bhattacharya S., Diamond L. S. Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates. Infect Immun. 1990 Oct;58(10):3458–3461. doi: 10.1128/iai.58.10.3458-3461.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blakely P., Sargeaunt P. G., Reed S. L. An immunogenic 30-kDa surface antigen of pathogenic clinical isolates of Entamoeba histolytica. J Infect Dis. 1990 Oct;162(4):949–954. doi: 10.1093/infdis/162.4.949. [DOI] [PubMed] [Google Scholar]
  5. Borst P. Molecular genetics of antigenic variation. Immunol Today. 1991 Mar;12(3):A29–A33. doi: 10.1016/S0167-5699(05)80009-X. [DOI] [PubMed] [Google Scholar]
  6. Diamond L. S. A new liquid medium for xenic cultivation of Entamoeba histolytica and other lumen-dwelling protozoa. J Parasitol. 1982 Oct;68(5):958–959. [PubMed] [Google Scholar]
  7. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  8. Dunne D. W. Schistosome carbohydrates. Parasitol Today. 1990 Feb;6(2):45–48. doi: 10.1016/0169-4758(90)90068-f. [DOI] [PubMed] [Google Scholar]
  9. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  10. Gillin F. D., Diamond L. S. Clonal growth of Entamoeba histolytica and other species of Entamoeba in agar. J Protozool. 1978 Nov;25(4):539–543. doi: 10.1111/j.1550-7408.1978.tb04182.x. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Mendis K. N., David P. H., Carter R. Antigenic polymorphism in malaria: is it an important mechanism for immune evasion? Immunol Today. 1991 Mar;12(3):A34–A37. doi: 10.1016/S0167-5699(05)80010-6. [DOI] [PubMed] [Google Scholar]
  13. Mirelman D. Ameba-bacterium relationship in amebiasis. Microbiol Rev. 1987 Jun;51(2):272–284. doi: 10.1128/mr.51.2.272-284.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mirelman D., Bracha R., Wexler A., Chayen A. Changes in isoenzyme patterns of a cloned culture of nonpathogenic Entamoeba histolytica during axenization. Infect Immun. 1986 Dec;54(3):827–832. doi: 10.1128/iai.54.3.827-832.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PHILLIPS B. P., WOLFE P. A., BARTGIS I. L. Studies on the ameba-bacteria relationship in amebiasis. II. Some concepts on the etiology of the disease. Am J Trop Med Hyg. 1958 Jul;7(4):392–399. doi: 10.4269/ajtmh.1958.7.392. [DOI] [PubMed] [Google Scholar]
  16. PHILLIPS B. P., WOLFE P. A., REES C. W., GORDON H. A., WRIGHT W. H., REYNIERS J. A. Studies on the ameba-bacteria relationship in amebiasis; comparative results of the intracecal inoculation of germfree, monocontaminated, and conventional guinea pigs with Entamoeba histolytica. Am J Trop Med Hyg. 1955 Jul;4(4):675–692. [PubMed] [Google Scholar]
  17. Phillips B. P. Entamoeba histolytica: concurrent irreversible loss of infectivity-pathogenicity and encystment potential after prolonged maintenance in axenic culture in vitro. Exp Parasitol. 1973 Oct;34(2):163–167. doi: 10.1016/0014-4894(73)90075-1. [DOI] [PubMed] [Google Scholar]
  18. Pillai S., Mohimen A. A solid-phase sandwich radioimmunoassay for Entamoeba histolytica proteins and the detection of circulating antigens in amoebiasis. Gastroenterology. 1982 Dec;83(6):1210–1216. [PubMed] [Google Scholar]
  19. Sacks D. L., Hieny S., Sher A. Identification of cell surface carbohydrate and antigenic changes between noninfective and infective developmental stages of Leishmania major promastigotes. J Immunol. 1985 Jul;135(1):564–569. [PubMed] [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walsh J. A. Amebiasis in the world. Arch Invest Med (Mex) 1986;17 (Suppl 1):385–389. [PubMed] [Google Scholar]
  22. Wittner M., Rosenbaum R. M. Role of bacteria in modifying virulence of Entamoeba histolytica. Studies of amebae from axenic cultures. Am J Trop Med Hyg. 1970 Sep;19(5):755–761. doi: 10.4269/ajtmh.1970.19.755. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES