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. 2008 Nov;10(11):1204–1212. doi: 10.1593/neo.08642

Figure 3.

Figure 3

Suppression of merlin causes loss of contact-dependent inhibition of growth and promotes anchorage-independent growth. (A) Growth curves in the presence and absence of merlin expression. Cultures were subconfluent during the first 6 days. MENII-1-NF2-siRNA cells (dotted lines) continued growing after confluent conditions and have less contact-dependent inhibition of growth compared with MENII-1-Control cells (solid lines). Each line corresponds to representative cultures. (B) NF2 suppression promotes anchorage-independent growth. Marked increased in colony formation in soft agar was observed in cells without merlin expression as MENII-1-NF2-siRNA and KT21MG1-Control cells compared with MENII-1-Control and KT21MG1-NF2, respectively. Representative images of the colonies formed (upper panel) and the mean number of colonies per well (lower panel) are shown. Error bars equal ±SE of three independent experiments. Asterisks denote statistical significance (P < .05).