Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Jun;60(6):2551–2553. doi: 10.1128/iai.60.6.2551-2553.1992

Cell envelope proteins of Staphylococcus epidermidis grown in vivo in a peritoneal chamber implant.

B Modun 1, P Williams 1, W J Pike 1, A Cockayne 1, J P Arbuthnott 1, R Finch 1, S P Denyer 1
PMCID: PMC257197  PMID: 1587623

Abstract

Staphylococcus epidermidis was grown in vivo in chambers implanted intraperitoneally in rats. The cell wall and cytoplasmic membrane protein profiles of the in vivo-grown organisms were compared with those of S. epidermidis grown in vitro in nutrient broth (NB), in iron-restricted NB, or in pooled human peritoneal dialysate (HPD). Compared with growth in broth and in common with growth in HPD, growth in vivo in chambers resulted in the repression of many S. epidermidis wall proteins, with proteins of 27, 42, 54, and 70 kDa predominating. Growth in vivo also resulted in the induction of two iron-repressible cytoplasmic membrane proteins of 32 and 36 kDa, which were also present in staphylococci grown in HPD and in iron-restricted NB. Immunoblotting experiments revealed that in sera taken 21 days after inoculation of the intraperitoneal chambers, the predominant antibody response to cell envelope proteins was directed against the 32- and 36-kDa iron-repressible membrane proteins.

Full text

PDF
2551

Images in this article

2552Image
on p.2552
2552Image
on p.2552
2553Image
on p.2553

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown M. R., Williams P. The influence of environment on envelope properties affecting survival of bacteria in infections. Annu Rev Microbiol. 1985;39:527–556. doi: 10.1146/annurev.mi.39.100185.002523. [DOI] [PubMed] [Google Scholar]
  2. Fry R. J., Sinclair W. K. New dosimetry of atomic bomb radiations. Lancet. 1987 Oct 10;2(8563):845–848. doi: 10.1016/s0140-6736(87)91027-0. [DOI] [PubMed] [Google Scholar]
  3. Patrick C. C., Plaunt M. R., Sweet S. M., Patrick G. S. Defining Staphylococcus epidermidis cell wall proteins. J Clin Microbiol. 1990 Dec;28(12):2757–2760. doi: 10.1128/jcm.28.12.2757-2760.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pike W. J., Cockayne A., Webster C. A., Slack R. C., Shelton A. P., Arbuthnott J. P. Development and design of a novel in vivo chamber implant for the analysis of microbial virulence and assessment of antimicrobial therapy. Microb Pathog. 1991 Jun;10(6):443–450. doi: 10.1016/0882-4010(91)90109-n. [DOI] [PubMed] [Google Scholar]
  5. Shand G. H., Anwar H., Kadurugamuwa J., Brown M. R., Silverman S. H., Melling J. In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun. 1985 Apr;48(1):35–39. doi: 10.1128/iai.48.1.35-39.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smith D. G., Wilcox M. H., Williams P., Finch R. G., Denyer S. P. Characterization of cell envelope proteins of Staphylococcus epidermidis cultured in human peritoneal dialysate. Infect Immun. 1991 Feb;59(2):617–624. doi: 10.1128/iai.59.2.617-624.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Spencer R. C. Infections in continuous ambulatory peritoneal dialysis. J Med Microbiol. 1988 Sep;27(1):1–9. doi: 10.1099/00222615-27-1-1. [DOI] [PubMed] [Google Scholar]
  8. Verbrugh H. A., Keane W. F., Conroy W. E., Peterson P. K. Bacterial growth and killing in chronic ambulatory peritoneal dialysis fluids. J Clin Microbiol. 1984 Aug;20(2):199–203. doi: 10.1128/jcm.20.2.199-203.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ward K. H., Anwar H., Brown R. W., Wale J., Gowar J. Antibody response to outer-membrane antigens of Pseudomonas aeruginosa in human burn wound infection. J Med Microbiol. 1988 Nov;27(3):179–190. doi: 10.1099/00222615-27-3-179. [DOI] [PubMed] [Google Scholar]
  10. Wilcox M. H., Smith D. G., Evans J. A., Denyer S. P., Finch R. G., Williams P. Influence of carbon dioxide on growth and antibiotic susceptibility of coagulase-negative staphylococci cultured in human peritoneal dialysate. J Clin Microbiol. 1990 Oct;28(10):2183–2186. doi: 10.1128/jcm.28.10.2183-2186.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wilcox M. H., Williams P., Smith D. G., Modun B., Finch R. G., Denyer S. P. Variation in the expression of cell envelope proteins of coagulase-negative staphylococci cultured under iron-restricted conditions in human peritoneal dialysate. J Gen Microbiol. 1991 Nov;137(11):2561–2570. doi: 10.1099/00221287-137-11-2561. [DOI] [PubMed] [Google Scholar]
  12. Williams P. Role of the cell envelope in bacterial adaptation to growth in vivo in infections. Biochimie. 1988 Aug;70(8):987–1011. doi: 10.1016/0300-9084(88)90263-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES