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. 1992 Jun;60(6):2554–2558. doi: 10.1128/iai.60.6.2554-2558.1992

Predominant expansion of V gamma 9/V delta 2 T cells in a tularemia patient.

T Sumida 1, T Maeda 1, H Takahashi 1, S Yoshida 1, F Yonaha 1, A Sakamoto 1, H Tomioka 1, T Koike 1, S Yoshida 1
PMCID: PMC257198  PMID: 1534075

Abstract

We describe a 58-year-old man with tularemia and expanding gamma delta T cells in his peripheral blood lymphocytes (PBL) (32.7% of total PBL). In the present work, we analyzed the T-cell receptor V gamma/V delta repertoire of these cells by making use of the polymerase chain reaction and flow cytometry and found that they were mostly CD4- CD8- CD3+ V gamma 9/V delta 2+. The sequence analysis of 16 cDNA clones encoding the V gamma 9-J region revealed that the V gamma 9-Jp combination was strikingly overrepresented but that the junctional (N) region was heterogeneous. This suggested that the gamma delta T cells in PBL from a patient with tularemia were polyclonally expanded.

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Selected References

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