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. 1992 Jul;60(7):2677–2682. doi: 10.1128/iai.60.7.2677-2682.1992

Immunoglobulin G2 confers protection against Borrelia burgdorferi infection in LSH hamsters.

J L Schmitz 1, R F Schell 1, S M Callister 1, S D Lovrich 1, S P Day 1, J E Coe 1
PMCID: PMC257220  PMID: 1612738

Abstract

We showed that immune serum and its immunoglobulin fractions, specifically immunoglobulin G2 (IgG2), could confer complete protection to irradiated hamsters challenged with the Lyme disease spirochete. Immune serum and its immunoglobulin fractions also killed Borrelia burgdorferi in vitro. Depletion of complement in vivo abrogated the ability of IgG2 to confer complete protection against B. burgdorferi. Furthermore, the majority of antibody reactivity directed against B. burgdorferi was found in the IgG2 fraction. These findings demonstrate that IgG2 plays an important role in acquired resistance against infection with B. burgdorferi. Additional studies are needed to determine the mechanism(s) by which B. burgdorferi evades host defenses despite the development of an effective borreliacidal antibody response.

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Selected References

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  1. Callister S. M., Case K. L., Agger W. A., Schell R. F., Johnson R. C., Ellingson J. L. Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi. J Clin Microbiol. 1990 Feb;28(2):363–365. doi: 10.1128/jcm.28.2.363-365.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coe J. E., Coe P. R., Ross M. J. Staphylococcal protein A purification of rodent IgG1 and IgG2 with particular emphasis on syrian hamsters. Mol Immunol. 1981 Nov;18(11):1007–1012. doi: 10.1016/0161-5890(81)90119-x. [DOI] [PubMed] [Google Scholar]
  3. Coe J. E. Humoral immunity and serum proteins in the Syrian hamster. Fed Proc. 1978 May 15;37(7):2030–2031. [PubMed] [Google Scholar]
  4. Coe J. E. The immune response in the hamster. I. Definition of two 7 S globulin classes: 7 S-gamma-1 and 7 S-gamma-2. J Immunol. 1968 Mar;100(3):507–515. [PubMed] [Google Scholar]
  5. Coe J. E. The immune response in the hamster. II. Studies on IgM. Immunology. 1970 Feb;18(2):223–236. [PMC free article] [PubMed] [Google Scholar]
  6. Coe J., Peel L., Smith R. F. The immune response in the hamster. V. Biologic activities of 7S-gamma-1 and 7S-gamma-2 globulins. J Immunol. 1971 Jul;107(1):76–82. [PubMed] [Google Scholar]
  7. Fikrig E., Barthold S. W., Kantor F. S., Flavell R. A. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science. 1990 Oct 26;250(4980):553–556. doi: 10.1126/science.2237407. [DOI] [PubMed] [Google Scholar]
  8. Hejka A., Schmitz J. L., England D. M., Callister S. M., Schell R. F. Histopathology of Lyme arthritis in LSH hamsters. Am J Pathol. 1989 May;134(5):1113–1123. [PMC free article] [PubMed] [Google Scholar]
  9. Johnson R. C., Kodner C. L., Russell M. E. Vaccination of hamsters against experimental infection with Borrelia burgdorferi. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):45–48. doi: 10.1016/s0176-6724(86)80101-8. [DOI] [PubMed] [Google Scholar]
  10. Johnson R. C., Kodner C., Russell M. Active immunization of hamsters against experimental infection with Borrelia burgdorferi. Infect Immun. 1986 Dec;54(3):897–898. doi: 10.1128/iai.54.3.897-898.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson R. C., Kodner C., Russell M., Duray P. H. Experimental infection of the hamster with Borrelia burgdorferi. Ann N Y Acad Sci. 1988;539:258–263. doi: 10.1111/j.1749-6632.1988.tb31859.x. [DOI] [PubMed] [Google Scholar]
  12. Johnson R. C., Kodner C., Russell M. Passive immunization of hamsters against experimental infection with the Lyme disease spirochete. Infect Immun. 1986 Sep;53(3):713–714. doi: 10.1128/iai.53.3.713-714.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson R. C., Marek N., Kodner C. Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol. 1984 Dec;20(6):1099–1101. doi: 10.1128/jcm.20.6.1099-1101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kochi S. K., Johnson R. C. Role of immunoglobulin G in killing of Borrelia burgdorferi by the classical complement pathway. Infect Immun. 1988 Feb;56(2):314–321. doi: 10.1128/iai.56.2.314-321.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lovrich S. D., Callister S. M., Schmitz J. L., Alder J. D., Schell R. F. Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete. Infect Immun. 1991 Aug;59(8):2522–2528. doi: 10.1128/iai.59.8.2522-2528.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Portis J. L., Coe J. E. The immune response in the hamster. VII. Studies on cytophilic immunoglobulin. J Immunol. 1975 Sep;115(3):693–700. [PubMed] [Google Scholar]
  18. Schmitz J. L., Lovrich S. D., Callister S. M., Schell R. F. Depletion of complement and effects on passive transfer of resistance to infection with Borrelia burgdorferi. Infect Immun. 1991 Oct;59(10):3815–3818. doi: 10.1128/iai.59.10.3815-3818.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmitz J. L., Schell R. F., Hejka A. G., England D. M. Passive immunization prevents induction of Lyme arthritis in LSH hamsters. Infect Immun. 1990 Jan;58(1):144–148. doi: 10.1128/iai.58.1.144-148.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schmitz J. L., Schell R. F., Hejka A., England D. M., Konick L. Induction of lyme arthritis in LSH hamsters. Infect Immun. 1988 Sep;56(9):2336–2342. doi: 10.1128/iai.56.9.2336-2342.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schmitz J. L., Schell R. F., Lovrich S. D., Callister S. M., Coe J. E. Characterization of the protective antibody response to Borrelia burgdorferi in experimentally infected LSH hamsters. Infect Immun. 1991 Jun;59(6):1916–1921. doi: 10.1128/iai.59.6.1916-1921.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schwan T. G., Kime K. K., Schrumpf M. E., Coe J. E., Simpson W. J. Antibody response in white-footed mice (Peromyscus leucopus) experimentally infected with the Lyme disease spirochete (Borrelia burgdorferi). Infect Immun. 1989 Nov;57(11):3445–3451. doi: 10.1128/iai.57.11.3445-3451.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steere A. C., Grodzicki R. L., Kornblatt A. N., Craft J. E., Barbour A. G., Burgdorfer W., Schmid G. P., Johnson E., Malawista S. E. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. doi: 10.1056/NEJM198303313081301. [DOI] [PubMed] [Google Scholar]
  24. Steiner B. M., Schell R. F., Harris O. N. The effect of C3 depletion on resistance of hamsters to infection with the yaws spirochete. Sex Transm Dis. 1986 Oct-Dec;13(4):245–250. doi: 10.1097/00007435-198610000-00007. [DOI] [PubMed] [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Winkelstein J. A. Complement and the host's defense against the pneumococcus. Crit Rev Microbiol. 1984;11(3):187–208. doi: 10.3109/10408418409105903. [DOI] [PubMed] [Google Scholar]

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