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. 1992 Jul;60(7):2995–2997. doi: 10.1128/iai.60.7.2995-2997.1992

Virulence-associated 15- to 17-kilodalton antigens in Rhodococcus equi: temperature-dependent expression and location of the antigens.

S Takai 1, M Iie 1, Y Watanabe 1, S Tsubaki 1, T Sekizaki 1
PMCID: PMC257265  PMID: 1612765

Abstract

Virulent Rhodococcus equi showing 15- to 17-kDa antigens, which is virulent in mice, was found to harbor an 85-kbp plasmid, and the 15- to 17-kDa antigens were found to be associated with possession of the 85-kbp plasmid of R. equi (S. Takai, T. Sekizaki, T. Ozawa, T. Sugawara, Y. Watanabe, and S. Tsubaki, Infect. Immun. 59:4056-4060, 1991). The expression of these antigens was temperature regulated: when cells were grown at a low temperature (25 to 32 degrees C), they did not express them, whereas they expressed them in large amounts when the cells were grown at a higher temperature (34 to 41 degrees C). The antigens were expressed on the cell surface, as evidenced by their susceptibility to proteolysis by a trypsin and by the biotin-avidin protein-blotting technique.

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Selected References

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