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. 1992 Jul;60(7):3002–3006. doi: 10.1128/iai.60.7.3002-3006.1992

Morphological and biochemical study of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha-toxin.

A Oksche 1, R Nakov 1, E Habermann 1
PMCID: PMC257267  PMID: 1612767

Abstract

Clostridium novyi alpha-toxin caused retraction and rounding of cultured endothelial cells from porcine pulmonary arteries; nevertheless, the endothelial cells firmly adhered to their supports. F-actin stained with fluorescein-labeled phalloidin was condensed around the nucleus, whereas intermediate filaments and microtubules appeared unchanged. The content of F-actin and myosin was decreased, but that of G-actin or vimentin was not. A predominant role of the microfilament system in C. novyi alpha-toxin cytopathic action is suggested.

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Selected References

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