Abstract
The signal transduction pathways by which staphylococcal toxic shock syndrome toxin 1 (TSST-1) induces tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) secretion were examined with various protein kinase inhibitors. TNF-alpha secretion by normal human monocytes and T cells in response to TSST-1 was suppressed by inhibitors of protein kinase C (H7) and tyrosine kinases (genistein). In contrast, the secretion of IL-1 beta was blocked by a cyclic AMP- and cyclic GMP-dependent kinase inhibitor (HA1004) as well as by H7 and genistein. These results suggest that the secretion of TNF-alpha and IL-1 beta may be differentially regulated by TSST-1 and that protein kinases play an important role in mediating cytokine responses to the toxin.
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Selected References
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- Bakouche O., Moreau J. L., Lachman L. B. Secretion of IL-1: role of protein kinase C. J Immunol. 1992 Jan 1;148(1):84–91. [PubMed] [Google Scholar]
- Chatila T. A., Schwartz D. H., Miller R., Geha R. S. Requirement for mitogen, T cell-accessory cell contact, and interleukin 1 in the induction of resting T-cell proliferation. Clin Immunol Immunopathol. 1987 Aug;44(2):235–247. doi: 10.1016/0090-1229(87)90068-7. [DOI] [PubMed] [Google Scholar]
- Choi Y. W., Kotzin B., Herron L., Callahan J., Marrack P., Kappler J. Interaction of Staphylococcus aureus toxin "superantigens" with human T cells. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8941–8945. doi: 10.1073/pnas.86.22.8941. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Boer M., Reijneke R., Van de Griend R. J., Loos J. A., Roos D. Large-scale purification and cryopreservation of human monocytes. J Immunol Methods. 1981;43(2):225–239. doi: 10.1016/0022-1759(81)90027-2. [DOI] [PubMed] [Google Scholar]
- Fast D. J., Schlievert P. M., Nelson R. D. Toxic shock syndrome-associated staphylococcal and streptococcal pyrogenic toxins are potent inducers of tumor necrosis factor production. Infect Immun. 1989 Jan;57(1):291–294. doi: 10.1128/iai.57.1.291-294.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hidaka H., Inagaki M., Kawamoto S., Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984 Oct 9;23(21):5036–5041. doi: 10.1021/bi00316a032. [DOI] [PubMed] [Google Scholar]
- Imamura K., Dianoux A., Nakamura T., Kufe D. Colony-stimulating factor 1 activates protein kinase C in human monocytes. EMBO J. 1990 Aug;9(8):2423-8, 2389. doi: 10.1002/j.1460-2075.1990.tb07418.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kappler J., Kotzin B., Herron L., Gelfand E. W., Bigler R. D., Boylston A., Carrel S., Posnett D. N., Choi Y., Marrack P. V beta-specific stimulation of human T cells by staphylococcal toxins. Science. 1989 May 19;244(4906):811–813. doi: 10.1126/science.2524876. [DOI] [PubMed] [Google Scholar]
- Kovacs E. J., Radzioch D., Young H. A., Varesio L. Differential inhibition of IL-1 and TNF-alpha mRNA expression by agents which block second messenger pathways in murine macrophages. J Immunol. 1988 Nov 1;141(9):3101–3105. [PubMed] [Google Scholar]
- Madsen M., Johnsen H. E. A methodological study of E-rosette formation using AET-treated sheep red blood cells. J Immunol Methods. 1979 May 10;27(1):61–74. doi: 10.1016/0022-1759(79)90239-4. [DOI] [PubMed] [Google Scholar]
- Marrack P., Kappler J. The staphylococcal enterotoxins and their relatives. Science. 1990 May 11;248(4956):705–711. doi: 10.1126/science.2185544. [DOI] [PubMed] [Google Scholar]
- Nezu N., Ryu K., Koide Y., Yoshida T. O. Regulation of HLA class II molecule expressions by IFN-gamma. The signal transduction mechanism in glioblastoma cell lines. J Immunol. 1990 Nov 1;145(9):3126–3135. [PubMed] [Google Scholar]
- Nishizuka Y., Takai Y., Kishimoto A., Kikkawa U., Kaibuchi K. Phospholipid turnover in hormone action. Recent Prog Horm Res. 1984;40:301–345. doi: 10.1016/b978-0-12-571140-1.50012-8. [DOI] [PubMed] [Google Scholar]
- Nishizuka Y. The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988 Aug 25;334(6184):661–665. doi: 10.1038/334661a0. [DOI] [PubMed] [Google Scholar]
- Parsonnet J., Gillis Z. A. Production of tumor necrosis factor by human monocytes in response to toxic-shock-syndrome toxin-1. J Infect Dis. 1988 Nov;158(5):1026–1033. doi: 10.1093/infdis/158.5.1026. [DOI] [PubMed] [Google Scholar]
- Parsonnet J., Hickman R. K., Eardley D. D., Pier G. B. Induction of human interleukin-1 by toxic-shock-syndrome toxin-1. J Infect Dis. 1985 Mar;151(3):514–522. doi: 10.1093/infdis/151.3.514. [DOI] [PubMed] [Google Scholar]
- Rosten P. M., Bartlett K. H., Chow A. W. Purification and purity assessment of toxic shock syndrome toxin 1. Rev Infect Dis. 1989 Jan-Feb;11 (Suppl 1):S110–S116. doi: 10.1093/clinids/11.supplement_1.s110. [DOI] [PubMed] [Google Scholar]
- See R. H., Kum W. W., Chang A. H., Goh S. H., Chow A. W. Induction of tumor necrosis factor and interleukin-1 by purified staphylococcal toxic shock syndrome toxin 1 requires the presence of both monocytes and T lymphocytes. Infect Immun. 1992 Jul;60(7):2612–2618. doi: 10.1128/iai.60.7.2612-2618.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taniguchi H., Sakano T., Hamasaki T., Kashiwa H., Ueda K. Effect of protein kinase C inhibitor (H-7) and calmodulin antagonist (W-7) on pertussis toxin-induced IL-1 production by human adherent monocytes. Comparison with lipopolysaccharide as a stimulator of IL-1 production. Immunology. 1989 Jun;67(2):210–215. [PMC free article] [PubMed] [Google Scholar]