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. 1992 Sep;60(9):3892–3896. doi: 10.1128/iai.60.9.3892-3896.1992

Immune CD4+ T cells specific for Theileria parva-infected lymphocytes recognize a 24-kilodalton protein.

D J Grab 1, C L Baldwin 1, W C Brown 1, E A Innes 1, J D Lonsdale-Eccles 1, Y Verjee 1
PMCID: PMC257404  PMID: 1354201

Abstract

Theileria parva is a protozoan parasite that infects and transforms bovine lymphocytes. Here we report the partial purification of a T. parva-specific protein from infected lymphocytes that is recognized by CD4+ parasite-specific T-cell clones derived from immune cattle. T. parva-infected lymphocytes were homogenized in Dulbecco's phosphate-buffered saline in the presence of protease inhibitors. The antigen was purified from a postmicrosomal supernatant by using a combination of DEAE-cellulose chromatography and hydroxylapatite column chromatography. After labelling with 125I, the antigen preparation was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain 8 to 10 proteins. This preparation was subjected to chromatography in phosphate-buffered saline on HPLC TSK-250/125 columns coupled in tandem. A radiolabelled protein of M(r) 24,000 correlated with antigenic activity.

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Selected References

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