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. 1992 Oct;60(10):4343–4349. doi: 10.1128/iai.60.10.4343-4349.1992

Effect of growth temperature on outer membrane components and virulence of Aeromonas hydrophila strains of serotype O:34.

S Merino 1, S Camprubí 1, J M Tomás 1
PMCID: PMC257471  PMID: 1398945

Abstract

Growth of Aeromonas hydrophila strains from serotype O:34 at 20 and 37 degrees C in tryptic soy broth resulted in changes in the lipids, lipopolysaccharide (LPS), and virulence of the strains tested. Cells grown at 20 degrees C contained, relative to those cultured at 37 degrees C, increased levels of the phospholipid fatty acids hexadecanoate and octadecanoate and reduced levels of the corresponding saturated fatty acids. Furthermore, the lipid A fatty acids also showed thermoadaptation. In addition, LPS extracted from cells cultivated at 20 degrees C was smooth, while the LPS extracted from the same cells cultivated at 37 degrees C was rough. Finally, the strains were more virulent for fish and mice when they were grown at 20 degrees C than when they were grown at 37 degrees C and also showed increased different extracellular activities when they were grown at 20 degrees C.

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Selected References

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