Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Oct;60(10):4418–4421. doi: 10.1128/iai.60.10.4418-4421.1992

Depletion of tryptophan is not involved in expression of tryptophanyl-tRNA synthetase mediated by interferon.

T Flohr 1, F C Bange 1, A von Euch 1, M Kiekenbeck 1, E C Böttger 1
PMCID: PMC257482  PMID: 1398953

Abstract

Gamma interferon (IFN-gamma) affects tryptophan metabolism by mediating the expression of indoleamine 2,3-dioxygenase and tryptophanyl-tRNA synthetase. In the present study, we investigated the role of indoleamine 2,3-dioxygenase-mediated tryptophan depletion in the induction of tryptophanyl-tRNA synthetase by IFN-gamma. The addition of excess tryptophan to the culture medium did not affect the induction of tryptophanyl-tRNA synthetase by IFN-gamma, indicating that tryptophan degradation is not directly involved in the IFN-gamma-mediated expression of tryptophanyl-tRNA synthetase.

Full text

PDF
4418

Images in this article

4419Image
on p.4419

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bange F. C., Flohr T., Buwitt U., Böttger E. C. An interferon-induced protein with release factor activity is a tryptophanyl-tRNA synthetase. FEBS Lett. 1992 Mar 30;300(2):162–166. doi: 10.1016/0014-5793(92)80187-l. [DOI] [PubMed] [Google Scholar]
  2. Buwitt U., Flohr T., Böttger E. C. Molecular cloning and characterization of an interferon induced human cDNA with sequence homology to a mammalian peptide chain release factor. EMBO J. 1992 Feb;11(2):489–496. doi: 10.1002/j.1460-2075.1992.tb05079.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Byrne G. I., Lehmann L. K., Landry G. J. Induction of tryptophan catabolism is the mechanism for gamma-interferon-mediated inhibition of intracellular Chlamydia psittaci replication in T24 cells. Infect Immun. 1986 Aug;53(2):347–351. doi: 10.1128/iai.53.2.347-351.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Böttger E. C., Blanar M. A., Flavell R. A. Cycloheximide, an inhibitor of protein synthesis, prevents gamma-interferon-induced expression of class II mRNA in a macrophage cell line. Immunogenetics. 1988;28(4):215–220. doi: 10.1007/BF00345497. [DOI] [PubMed] [Google Scholar]
  5. Caplen H. S., Gupta S. L. Differential regulation of a cellular gene by human interferon-gamma and interferon-alpha. J Biol Chem. 1988 Jan 5;263(1):332–339. [PubMed] [Google Scholar]
  6. Carlin J. M., Borden E. C., Byrne G. I. Interferon-induced indoleamine 2,3-dioxygenase activity inhibits Chlamydia psittaci replication in human macrophages. J Interferon Res. 1989 Jun;9(3):329–337. doi: 10.1089/jir.1989.9.329. [DOI] [PubMed] [Google Scholar]
  7. Dai W., Gupta S. L. Molecular cloning, sequencing and expression of human interferon-gamma-inducible indoleamine 2,3-dioxygenase cDNA. Biochem Biophys Res Commun. 1990 Apr 16;168(1):1–8. doi: 10.1016/0006-291x(90)91666-g. [DOI] [PubMed] [Google Scholar]
  8. Dai W., Gupta S. L. Regulation of indoleamine 2,3-dioxygenase gene expression in human fibroblasts by interferon-gamma. Upstream control region discriminates between interferon-gamma and interferon-alpha. J Biol Chem. 1990 Nov 15;265(32):19871–19877. [PubMed] [Google Scholar]
  9. Feng G. S., Taylor M. W. Interferon gamma-resistant mutants are defective in the induction of indoleamine 2,3-dioxygenase. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7144–7148. doi: 10.1073/pnas.86.18.7144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fleckner J., Rasmussen H. H., Justesen J. Human interferon gamma potently induces the synthesis of a 55-kDa protein (gamma 2) highly homologous to rabbit peptide chain release factor and bovine tryptophanyl-tRNA synthetase. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11520–11524. doi: 10.1073/pnas.88.24.11520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frolova LYu, Sudomoina M. A., Grigorieva AYu, Zinovieva O. L., Kisselev L. L. Cloning and nucleotide sequence of the structural gene encoding for human tryptophanyl-tRNA synthetase. Gene. 1991 Dec 30;109(2):291–296. doi: 10.1016/0378-1119(91)90624-k. [DOI] [PubMed] [Google Scholar]
  12. Garret M., Pajot B., Trézéguet V., Labouesse J., Merle M., Gandar J. C., Benedetto J. P., Sallafranque M. L., Alterio J., Gueguen M. A mammalian tryptophanyl-tRNA synthetase shows little homology to prokaryotic synthetases but near identity with mammalian peptide chain release factor. Biochemistry. 1991 Aug 6;30(31):7809–7817. doi: 10.1021/bi00245a021. [DOI] [PubMed] [Google Scholar]
  13. Lazard M., Mirande M., Waller J. P. Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction. FEBS Lett. 1987 May 25;216(1):27–30. doi: 10.1016/0014-5793(87)80750-0. [DOI] [PubMed] [Google Scholar]
  14. Lee C. C., Craigen W. J., Muzny D. M., Harlow E., Caskey C. T. Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetases. Proc Natl Acad Sci U S A. 1990 May;87(9):3508–3512. doi: 10.1073/pnas.87.9.3508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan S. D., Söll D. Regulation of the biosynthesis of aminoacid: tRNA ligases and of tRNA. Prog Nucleic Acid Res Mol Biol. 1978;21:181–207. doi: 10.1016/s0079-6603(08)60270-6. [DOI] [PubMed] [Google Scholar]
  16. Neihardt F. C., Parker J., McKeever W. G. Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells. Annu Rev Microbiol. 1975;29:215–250. doi: 10.1146/annurev.mi.29.100175.001243. [DOI] [PubMed] [Google Scholar]
  17. Ozaki Y., Edelstein M. P., Duch D. S. Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1242–1246. doi: 10.1073/pnas.85.4.1242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pestka S., Langer J. A., Zoon K. C., Samuel C. E. Interferons and their actions. Annu Rev Biochem. 1987;56:727–777. doi: 10.1146/annurev.bi.56.070187.003455. [DOI] [PubMed] [Google Scholar]
  19. Pfefferkorn E. R. Interferon gamma blocks the growth of Toxoplasma gondii in human fibroblasts by inducing the host cells to degrade tryptophan. Proc Natl Acad Sci U S A. 1984 Feb;81(3):908–912. doi: 10.1073/pnas.81.3.908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pfefferkorn E. R., Rebhun S., Eckel M. Characterization of an indoleamine 2,3-dioxygenase induced by gamma-interferon in cultured human fibroblasts. J Interferon Res. 1986 Jun;6(3):267–279. doi: 10.1089/jir.1986.6.267. [DOI] [PubMed] [Google Scholar]
  21. Rapoza P. A., Tahija S. G., Carlin J. P., Miller S. L., Padilla M. L., Byrne G. I. Effect of interferon on a primary conjunctival epithelial cell model of trachoma. Invest Ophthalmol Vis Sci. 1991 Oct;32(11):2919–2923. [PubMed] [Google Scholar]
  22. Reid L. E., Brasnett A. H., Gilbert C. S., Porter A. C., Gewert D. R., Stark G. R., Kerr I. M. A single DNA response element can confer inducibility by both alpha- and gamma-interferons. Proc Natl Acad Sci U S A. 1989 Feb;86(3):840–844. doi: 10.1073/pnas.86.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rubin B. Y., Anderson S. L., Xing L., Powell R. J., Tate W. P. Interferon induces tryptophanyl-tRNA synthetase expression in human fibroblasts. J Biol Chem. 1991 Dec 25;266(36):24245–24248. [PubMed] [Google Scholar]
  24. Shimizu T., Nomiyama S., Hirata F., Hayaishi O. Indoleamine 2,3-dioxygenase. Purification and some properties. J Biol Chem. 1978 Jul 10;253(13):4700–4706. [PubMed] [Google Scholar]
  25. Takikawa O., Kuroiwa T., Yamazaki F., Kido R. Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity. J Biol Chem. 1988 Feb 5;263(4):2041–2048. [PubMed] [Google Scholar]
  26. Taylor M. W., Feng G. S. Relationship between interferon-gamma, indoleamine 2,3-dioxygenase, and tryptophan catabolism. FASEB J. 1991 Aug;5(11):2516–2522. [PubMed] [Google Scholar]
  27. de la Maza L. M., Peterson E. M. Dependence of the in vitro antiproliferative activity of recombinant human gamma-interferon on the concentration of tryptophan in culture media. Cancer Res. 1988 Jan 15;48(2):346–350. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES