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. 1992 Oct;60(10):4439–4442. doi: 10.1128/iai.60.10.4439-4442.1992

Effect of exogenous sialylation of the lipooligosaccharide of Neisseria gonorrhoeae on opsonophagocytosis.

J J Kim 1, D Zhou 1, R E Mandrell 1, J M Griffiss 1
PMCID: PMC257487  PMID: 1398958

Abstract

Serum-sensitive Neisseria gonorrhoeae strains become serum resistant when grown in the presence of a sialic acid precursor, cytidine monophospho-N-acetylneuraminic acid. We examined the abilities of human neutrophils to phagocytose sialylated and nonsialylated gonococci and observed a decrease in the complement-dependent phagocytosis of sialylated gonococci compared with that of nonsialylated gonococci (50.7 versus 25.9% survival at 30 min). This decrease in opsonophagocytosis after sialylation may contribute to the pathogenicity of gonococcal infections.

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Selected References

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  1. Apicella M. A., Mandrell R. E., Shero M., Wilson M. E., Griffiss J. M., Brooks G. F., Lammel C., Breen J. F., Rice P. A. Modification by sialic acid of Neisseria gonorrhoeae lipooligosaccharide epitope expression in human urethral exudates: an immunoelectron microscopic analysis. J Infect Dis. 1990 Aug;162(2):506–512. doi: 10.1093/infdis/162.2.506. [DOI] [PubMed] [Google Scholar]
  2. Brown E. J. Complement receptors and phagocytosis. Curr Opin Immunol. 1991 Feb;3(1):76–82. doi: 10.1016/0952-7915(91)90081-b. [DOI] [PubMed] [Google Scholar]
  3. Fischer S. H., Rest R. F. Gonococci possessing only certain P.II outer membrane proteins interact with human neutrophils. Infect Immun. 1988 Jun;56(6):1574–1579. doi: 10.1128/iai.56.6.1574-1579.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Griffiss J. M., Jarvis G. A., O'Brien J. P., Eads M. M., Schneider H. Lysis of Neisseria gonorrhoeae initiated by binding of normal human IgM to a hexosamine-containing lipooligosaccharide epitope(s) is augmented by strain-specific, properdin-binding-dependent alternative complement pathway activation. J Immunol. 1991 Jul 1;147(1):298–305. [PubMed] [Google Scholar]
  5. Joiner K. A., Warren K. A., Brown E. J., Swanson J., Frank M. M. Studies on the mechanism of bacterial resistance to complement-mediated killing. IV. C5b-9 forms high molecular weight complexes with bacterial outer membrane constituents on serum-resistant but not on serum-sensitive Neisseria gonorrhoeae. J Immunol. 1983 Sep;131(3):1443–1451. [PubMed] [Google Scholar]
  6. Mandrell R. E., Griffiss J. M., Macher B. A. Lipooligosaccharides (LOS) of Neisseria gonorrhoeae and Neisseria meningitidis have components that are immunochemically similar to precursors of human blood group antigens. Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human erythrocytes. J Exp Med. 1988 Jul 1;168(1):107–126. doi: 10.1084/jem.168.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mandrell R. E., Kim J. J., John C. M., Gibson B. W., Sugai J. V., Apicella M. A., Griffiss J. M., Yamasaki R. Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis. J Bacteriol. 1991 May;173(9):2823–2832. doi: 10.1128/jb.173.9.2823-2832.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mandrell R. E., Lesse A. J., Sugai J. V., Shero M., Griffiss J. M., Cole J. A., Parsons N. J., Smith H., Morse S. A., Apicella M. A. In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation. J Exp Med. 1990 May 1;171(5):1649–1664. doi: 10.1084/jem.171.5.1649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nairn C. A., Cole J. A., Patel P. V., Parsons N. J., Fox J. E., Smith H. Cytidine 5'-monophospho-N-acetylneuraminic acid or a related compound is the low Mr factor from human red blood cells which induces gonococcal resistance to killing by human serum. J Gen Microbiol. 1988 Dec;134(12):3295–3306. doi: 10.1099/00221287-134-12-3295. [DOI] [PubMed] [Google Scholar]
  10. Parsons N. J., Patel P. V., Tan E. L., Andrade J. R., Nairn C. A., Goldner M., Cole J. A., Smith H. Cytidine 5'-monophospho-N-acetyl neuraminic acid and a low molecular weight factor from human blood cells induce lipopolysaccharide alteration in gonococci when conferring resistance to killing by human serum. Microb Pathog. 1988 Oct;5(4):303–309. doi: 10.1016/0882-4010(88)90103-9. [DOI] [PubMed] [Google Scholar]
  11. Rest R. F., Frangipane J. V. Growth of Neisseria gonorrhoeae in CMP-N-acetylneuraminic acid inhibits nonopsonic (opacity-associated outer membrane protein-mediated) interactions with human neutrophils. Infect Immun. 1992 Mar;60(3):989–997. doi: 10.1128/iai.60.3.989-997.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rest R. F., Shafer W. M. Interactions of Neisseria gonorrhoeae with human neutrophils. Clin Microbiol Rev. 1989 Apr;2 (Suppl):S83–S91. doi: 10.1128/cmr.2.suppl.s83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ross S. C., Densen P. Opsonophagocytosis of Neisseria gonorrhoeae: interaction of local and disseminated isolates with complement and neutrophils. J Infect Dis. 1985 Jan;151(1):33–41. doi: 10.1093/infdis/151.1.33. [DOI] [PubMed] [Google Scholar]
  14. Schneider H., Griffiss J. M., Williams G. D., Pier G. B. Immunological basis of serum resistance of Neisseria gonorrhoeae. J Gen Microbiol. 1982 Jan;128(1):13–22. doi: 10.1099/00221287-128-1-13. [DOI] [PubMed] [Google Scholar]
  15. Schoolnik G. K., Ochs H. D., Buchanan T. M. Immunoglobulin class responsible for gonococcal bactericidal activity of normal human sera. J Immunol. 1979 May;122(5):1771–1779. [PubMed] [Google Scholar]
  16. Swanson J. Colony opacity and protein II compositions of gonococci. Infect Immun. 1982 Jul;37(1):359–368. doi: 10.1128/iai.37.1.359-368.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tramont E. C., Sadoff J. C., Wilson C. Variability of the lytic susceptibility of Neisseria gonorrhoeae to human sera. J Immunol. 1977 May;118(5):1843–1851. [PubMed] [Google Scholar]

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