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. 1992 Jan;60(1):308–311. doi: 10.1128/iai.60.1.308-311.1992

Cell-free system responsible for internal radiolabeling of glycopeptidolipids of the Mycobacterium avium complex.

N Ramasesh 1, E L Wright 1, W W Barrow 1
PMCID: PMC257537  PMID: 1729193

Abstract

Internal radiolabeling of serotype-specific glycopeptidolipids with [14C]mannose was accomplished with a cell-free system derived from serotype 20 of the Mycobacterium avium complex. Similar radiolabeling was not apparent with a cell-free system derived from the rough colony variant, previously shown to be devoid of glycopeptidolipids. Although a comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis of the parent and rough variant strains revealed a close similarity, there were some proteins unique to the parent strain.

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Selected References

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