Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Mar;60(3):845–852. doi: 10.1128/iai.60.3.845-852.1992

Identification and characterization of lipopolysaccharide-binding proteins on human peripheral blood cell populations.

J L Halling 1, D R Hamill 1, M G Lei 1, D C Morrison 1
PMCID: PMC257564  PMID: 1371770

Abstract

Previous research in this laboratory, using photoactivatable radioiodinated lipopolysaccharide derivatized with sulfosuccinimidyl-2-(p-azidosalicylamide)-1,3'-dithiopropionate (125I-ASD-LPS), has resulted in the identification of a specific LPS receptor with a molecular mass of approximately 73 kDa on murine lymphocytes and splenic macrophages. The experiments presented in this report investigated whether a similar LPS-binding protein was also expressed on human peripheral blood populations, including monocytes, lymphocytes, neutrophils, platelets, and erythrocytes. Each cell population was incubated with 125I-ASD-LPS, UV irradiated, washed, reduced, and solubilized, and the cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography. On all of the cell populations, except erythrocytes, a similar 73-kDa LPS-binding protein was present. In addition, each population also expressed lower-molecular-weight secondary LPS-binding proteins, some of which were conserved among the populations. Binding of the photoactivatable LPS probe was found to be both time and temperature dependent. These data support the concept that the 73-kDa LPS-binding protein is conserved on multiple cell types from a variety of species.

Full text

PDF
845

Images in this article

847Image
on p.847
847Image
on p.847
848Image
on p.848
848Image
on p.848
848Image
on p.848
849Image
on p.849

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amrein P. C., Stossel T. P. Prevention of degradation of human polymorphonuclear leukocyte proteins by diisopropylfluorophosphate. Blood. 1980 Sep;56(3):442–447. [PubMed] [Google Scholar]
  2. Bright S. W., Chen T. Y., Flebbe L. M., Lei M. G., Morrison D. C. Generation and characterization of hamster-mouse hybridomas secreting monoclonal antibodies with specificity for lipopolysaccharide receptor. J Immunol. 1990 Jul 1;145(1):1–7. [PubMed] [Google Scholar]
  3. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen T. Y., Bright S. W., Pace J. L., Russell S. W., Morrison D. C. Induction of macrophage-mediated tumor cytotoxicity by a hamster monoclonal antibody with specificity for lipopolysaccharide receptor. J Immunol. 1990 Jul 1;145(1):8–12. [PubMed] [Google Scholar]
  5. Dziarski R. Peptidoglycan and lipopolysaccharide bind to the same binding site on lymphocytes. J Biol Chem. 1991 Mar 15;266(8):4719–4725. [PubMed] [Google Scholar]
  6. Gery I., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J Exp Med. 1972 Jul 1;136(1):143–155. doi: 10.1084/jem.136.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guthrie L. A., McPhail L. C., Henson P. M., Johnston R. B., Jr Priming of neutrophils for enhanced release of oxygen metabolites by bacterial lipopolysaccharide. Evidence for increased activity of the superoxide-producing enzyme. J Exp Med. 1984 Dec 1;160(6):1656–1671. doi: 10.1084/jem.160.6.1656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hampton R. Y., Golenbock D. T., Raetz C. R. Lipid A binding sites in membranes of macrophage tumor cells. J Biol Chem. 1988 Oct 15;263(29):14802–14807. [PubMed] [Google Scholar]
  9. Hara-Kuge S., Amano F., Nishijima M., Akamatsu Y. Isolation of a lipopolysaccharide (LPS)-resistant mutant, with defective LPS binding, of cultured macrophage-like cells. J Biol Chem. 1990 Apr 25;265(12):6606–6610. [PubMed] [Google Scholar]
  10. Havell E. A., Spitalny G. L. Endotoxin-induced interferon synthesis in macrophage cultures. J Reticuloendothel Soc. 1983 May;33(5):369–380. [PubMed] [Google Scholar]
  11. Hawiger J., Hawiger A., Timmons S. Endotoxin-sensitive membrane component of human platelets. Nature. 1975 Jul 10;256(5513):125–127. doi: 10.1038/256125a0. [DOI] [PubMed] [Google Scholar]
  12. Ispahani P., Pearson N. J., Greenwood D. An analysis of community and hospital-acquired bacteraemia in a large teaching hospital in the United Kingdom. Q J Med. 1987 May;63(241):427–440. [PubMed] [Google Scholar]
  13. Kirkland T. N., Virca G. D., Kuus-Reichel T., Multer F. K., Kim S. Y., Ulevitch R. J., Tobias P. S. Identification of lipopolysaccharide-binding proteins in 70Z/3 cells by photoaffinity cross-linking. J Biol Chem. 1990 Jun 5;265(16):9520–9525. [PubMed] [Google Scholar]
  14. Kreger B. E., Craven D. E., Carling P. C., McCabe W. R. Gram-negative bacteremia. III. Reassessment of etiology, epidemiology and ecology in 612 patients. Am J Med. 1980 Mar;68(3):332–343. doi: 10.1016/0002-9343(80)90101-1. [DOI] [PubMed] [Google Scholar]
  15. Lebbar S., Cavaillon J. M., Caroff M., Ledur A., Brade H., Sarfati R., Haeffner-Cavaillon N. Molecular requirement for interleukin 1 induction by lipopolysaccharide-stimulated human monocytes: involvement of the heptosyl-2-keto-3-deoxyoctulosonate region. Eur J Immunol. 1986 Jan;16(1):87–91. doi: 10.1002/eji.1830160117. [DOI] [PubMed] [Google Scholar]
  16. Lei M. G., Chen T. Y., Morrison D. C. Lipopolysaccharide/lipid A receptors on lymphocytes and macrophages. Int Rev Immunol. 1990;6(4):223–235. doi: 10.3109/08830189009056633. [DOI] [PubMed] [Google Scholar]
  17. Lei M. G., Morrison D. C. Specific endotoxic lipopolysaccharide-binding proteins on murine splenocytes. I. Detection of lipopolysaccharide-binding sites on splenocytes and splenocyte subpopulations. J Immunol. 1988 Aug 1;141(3):996–1005. [PubMed] [Google Scholar]
  18. Lei M. G., Morrison D. C. Specific endotoxic lipopolysaccharide-binding proteins on murine splenocytes. II. Membrane localization and binding characteristics. J Immunol. 1988 Aug 1;141(3):1006–1011. [PubMed] [Google Scholar]
  19. Lei M. G., Stimpson S. A., Morrison D. C. Specific endotoxic lipopolysaccharide-binding receptors on murine splenocytes. III. Binding specificity and characterization. J Immunol. 1991 Sep 15;147(6):1925–1932. [PubMed] [Google Scholar]
  20. McGhee J. R., Farrar J. J., Michalek S. M., Mergenhagen S. E., Rosenstreich D. L. Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens. J Exp Med. 1979 Apr 1;149(4):793–807. doi: 10.1084/jem.149.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Milner E. C., Rudbach J. A., Voneschen K. B. Cellular responses to bacterial lipopolysaccharide: T cells recognize LPS determinants. Scand J Immunol. 1983 Jul;18(1):21–28. doi: 10.1111/j.1365-3083.1983.tb00831.x. [DOI] [PubMed] [Google Scholar]
  22. Mita A., Ohta H., Mita T. Induction of splenic T cell proliferation by lipid A in mice immunized with sheep red blood cells. J Immunol. 1982 Apr;128(4):1709–1711. [PubMed] [Google Scholar]
  23. Miyazawa K., Mantel C., Lu L., Morrison D. C., Broxmeyer H. E. Lactoferrin-lipopolysaccharide interactions. Effect on lactoferrin binding to monocyte/macrophage-differentiated HL-60 cells. J Immunol. 1991 Jan 15;146(2):723–729. [PubMed] [Google Scholar]
  24. Morrison D. C., Leive L. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975 Apr 25;250(8):2911–2919. [PubMed] [Google Scholar]
  25. Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
  26. Morrison D. C., Ryan J. L. Endotoxins and disease mechanisms. Annu Rev Med. 1987;38:417–432. doi: 10.1146/annurev.me.38.020187.002221. [DOI] [PubMed] [Google Scholar]
  27. Morrison D. C. The case for specific lipopolysaccharide receptors expressed on mammalian cells. Microb Pathog. 1989 Dec;7(6):389–398. doi: 10.1016/0882-4010(89)90019-3. [DOI] [PubMed] [Google Scholar]
  28. Munford R. S., Hall C. L. Purification of acyloxyacyl hydrolase, a leukocyte enzyme that removes secondary acyl chains from bacterial lipopolysaccharides. J Biol Chem. 1989 Sep 15;264(26):15613–15619. [PubMed] [Google Scholar]
  29. Nagayama M., Zucker M. B., Beller F. K. Effects of a variety of endotoxins on human and rabbit platelet function. Thromb Diath Haemorrh. 1971 Dec 31;26(3):467–473. [PubMed] [Google Scholar]
  30. Ohno N., Morrison D. C. Lipopolysaccharide interaction with lysozyme. Binding of lipopolysaccharide to lysozyme and inhibition of lysozyme enzymatic activity. J Biol Chem. 1989 Mar 15;264(8):4434–4441. [PubMed] [Google Scholar]
  31. REAM V. J., DEYKIN D., GUREWICH V., WESSLER S. THE AGGREGATION OF HUMAN PLATELETS BY BACTERIAL ENDOTOXIN. J Lab Clin Med. 1965 Aug;66:245–252. [PubMed] [Google Scholar]
  32. Rietschel E. T., Brade L., Brandenburg K., Flad H. D., de Jong-Leuveninck J., Kawahara K., Lindner B., Loppnow H., Lüderitz T., Schade U. Chemical structure and biologic activity of bacterial and synthetic lipid A. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S527–S536. doi: 10.1093/clinids/9.supplement_5.s527. [DOI] [PubMed] [Google Scholar]
  33. Roeder D. J., Lei M. G., Morrison D. C. Endotoxic-lipopolysaccharide-specific binding proteins on lymphoid cells of various animal species: association with endotoxin susceptibility. Infect Immun. 1989 Apr;57(4):1054–1058. doi: 10.1128/iai.57.4.1054-1058.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tosato G., Seamon K. B., Goldman N. D., Sehgal P. B., May L. T., Washington G. C., Jones K. D., Pike S. E. Monocyte-derived human B-cell growth factor identified as interferon-beta 2 (BSF-2, IL-6). Science. 1988 Jan 29;239(4839):502–504. doi: 10.1126/science.2829354. [DOI] [PubMed] [Google Scholar]
  35. Tracey K. J., Lowry S. F., Cerami A. Cachectin/TNF mediates the pathophysiological effects of bacterial endotoxin/lipopolysaccharide (LPS). Prog Clin Biol Res. 1988;272:77–88. [PubMed] [Google Scholar]
  36. Weiss J., Muello K., Victor M., Elsbach P. The role of lipopolysaccharides in the action of the bactericidal/permeability-increasing neutrophil protein on the bacterial envelope. J Immunol. 1984 Jun;132(6):3109–3115. [PubMed] [Google Scholar]
  37. Wollenweber H. W., Morrison D. C. Synthesis and biochemical characterization of a photoactivatable, iodinatable, cleavable bacterial lipopolysaccharide derivative. J Biol Chem. 1985 Dec 5;260(28):15068–15074. [PubMed] [Google Scholar]
  38. Wright G. G., Read P. W., Mandell G. L. Lipopolysaccharide releases a priming substance from platelets that augments the oxidative response of polymorphonuclear neutrophils to chemotactic peptide. J Infect Dis. 1988 Apr;157(4):690–696. doi: 10.1093/infdis/157.4.690. [DOI] [PubMed] [Google Scholar]
  39. Wright S. D., Jong M. T. Adhesion-promoting receptors on human macrophages recognize Escherichia coli by binding to lipopolysaccharide. J Exp Med. 1986 Dec 1;164(6):1876–1888. doi: 10.1084/jem.164.6.1876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Wright S. D., Ramos R. A., Tobias P. S., Ulevitch R. J., Mathison J. C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990 Sep 21;249(4975):1431–1433. doi: 10.1126/science.1698311. [DOI] [PubMed] [Google Scholar]
  41. Wright S. D., Tobias P. S., Ulevitch R. J., Ramos R. A. Lipopolysaccharide (LPS) binding protein opsonizes LPS-bearing particles for recognition by a novel receptor on macrophages. J Exp Med. 1989 Oct 1;170(4):1231–1241. doi: 10.1084/jem.170.4.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES